Production of oligosaccharides from polysaccharides

ABSTRACT

Synthetic oligosaccharides and mixtures thereof which resemble polysaccharides produced by plants, yeast and other fungi, bacteria, and algae are described. The oligosaccharides and compositions provided herein are useful as synbiotics, prebiotics, immune modulators, digestion aids, and food additives.

CROSS-REFERENCES TO RELATED APPLICATIONS

This application claims priority to U.S. Provisional Application No. 62/857,685, filed Jun. 5, 2019, and U.S. Provisional Application No. 62/950,483, filed Dec. 19, 2019, the disclosures of which are hereby incorporated by reference in their entirety for all purposes.

BACKGROUND OF THE INVENTION

Oligosaccharides are carbohydrates that generally contain 3-20 covalently linked monosaccharide units. Due to the possibility of many monosaccharide constituents, variable glycosidic bond positions and associated stereochemistry, branching, and functional group modification, oligosaccharides can have highly variable structures that elicit a host of different bioactivities. The bioactivity of many oligosaccharides has not yet been studied or determined. However, some sources of oligosaccharides, particularly those in human milk¹⁻⁴, bovine milk,⁵ and galactooligosaccharides produced by the trans-galactosylation of lactose⁶, are well studied and have shown to act as prebiotics, immunomodulators, anti-diarrheal, pathogen protectants, and mineral absorption stimulators. Oligosaccharides from other sources are therefore expected to elicit similar bioactivities. Polysaccharides are large biomolecules that reach up to and possibly over 1 million monosaccharide residues.⁷ Polysaccharides make up the cell walls of plants, bacteria, yeast, fungi, and in a more limited manner, animals.⁸⁻⁹ Polysaccharides are largely known for their physical and rheological properties, which include providing structural rigidity, water binding, and antioxidant capabilities. Conversely, the bioactivities of polysaccharides are much less understood than oligosaccharides, but, in some cases, can provide similar prebiotic and immunomodulating benefits.¹⁰⁻¹² The difference in bioactivities despite similar structural components can be attributed to their low solubility and their limited intracellular transport.

While oligosaccharides can be found in high abundance in animals, the majority of plant matter is comprised of polysaccharides with little to no oligosaccharide contribution. Polysaccharides are generally synthesized by the addition of UDP-activated monosaccharides to carbohydrate primer material that is often protein or lipid bound.¹³⁻¹⁴ This leaves no direct mechanism for oligosaccharide biosynthesis, which results in few endogenous oligosaccharides. The most abundant plant polysaccharides found in nature include amylose, amylopectin, and cellulose. However, plants contain a number of other polysaccharides that can be grouped based upon their structural functions. Pectins refer to negatively charged anionic polymers such as rhamnogalacturonan I, rhamnogalacturonan II, and polygalacturonan, while hemicelluloses refer to neutral polymers that bind together cellulose fibrils such as xyloglucan, glucomannan, and arabinogalactan. Additionally, plant secondary cell walls largely contain arabinans and xylans.¹⁵⁻¹⁷

Oligosaccharides are generally produced in three ways and are subsequently ordered by the frequency of use: 1) de novo synthesis using chemical and/or enzymatic and/or biosynthetic approaches to construct oligosaccharides¹⁸⁻²⁰, 2) enzymatic or chemical release from proteins and/or lipids²¹, 3) through the depolymerization of polysaccharides using enzymes²². Due to the high development cost of the de novo synthesis of oligosaccharides, this strategy is currently only being applied to mammalian oligosaccharides. Additionally, plants do not have high amounts of protein and lipid bound oligosaccharides that can be released. Accordingly, the depolymerization of polysaccharides using enzymes is currently the primary way of producing plant oligosaccharides. However, the substrate specificity of enzymes leads to the production of highly specific oligosaccharides with little variation in the structures. Furthermore, enzymes are not readily available for all polysaccharides.

BRIEF SUMMARY OF THE INVENTION

Provided herein are synthetic oligosaccharides and mixtures thereof which resemble polysaccharides produced by plants, yeast and other fungi, bacteria, and algae are described.

In some embodiments, the synthetic oligosaccharide includes a backbone containing glucose monomers, wherein each glucose monomer is optionally bonded to a pendant xylose monomer. In some embodiments, the synthetic oligosaccharide includes a backbone containing mannose monomers, wherein each mannose monomer is optionally bonded to a pendant galactose monomer. In some embodiments, the synthetic oligosaccharide contains mannose monomers and glucose monomers. In some embodiments, the synthetic oligosaccharide includes a backbone containing arabinose monomers, wherein each arabinose monomer is optionally bonded to a pendant arabinose monomer. In some embodiments, the synthetic oligosaccharide includes β1-4 linked glucose monomers and β1-3 linked glucose monomers. In some embodiments, the synthetic oligosaccharide includes a backbone containing xylose monomers, wherein each xylose monomer is optionally bonded to a pendant arabinose monomer or a pendant glucuronic acid (e.g., a 4-O methylated GlcA). In some embodiments, the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30.

The oligosaccharides and compositions provided herein are useful as synbiotics, prebiotics, immune modulators, digestion aids, food additives, supplements, pharmaceutical excipients, and analytical standards.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A-1C show a) Annotated base peak chromatogram of characterized xyloglucan-derived oligosaccharides; b) Extracted ion chromatogram of 771.27 m/z shows four isomers; c) Annotated tandem mass spectra of the four 771.27 m/z isomers and their final elucidated structures.

FIGS. 2A-2N show annotated base peak chromatograms of oligosaccharides derived from polysaccharide standards: a) curdlan, b) cellulose, c) β-glucan, d) lichenan, e) galactan, f) mannan, g) glucomannan, h) galactomannan, i) arabinan, j) xylan, k) arabinoxylan, l) amylose, m) amylopectin, and n) xyloglucan.

FIG. 3 shows a) annotated base peak chromatogram of pooled oligosaccharides derived from the depolymerization of the polysaccharide standards; and annotated base peak chromatograms of characterized oligosaccharides derived from b) wheat bran and c) oat bran.

FIG. 4 shows a representation of the xyloglucan polysaccharide. The degree of polymerization can reach up to 100,000 monosaccharides.

FIG. 5 shows The base peak chromatogram for the xyloglucan resembling oligosaccharides. The chromatogram was extracted for masses between m/z 400-3000 with the calibrant ion m/z 922.00 excluded. The labeled oligosaccharides correspond to the oligosaccharides described in Table 4.

FIGS. 6A-6D show a) The fragmentation spectra of m/z 477.19 at retention time 11.04 minutes. This oligosaccharide represents #3 in Table 4. b) The fragmentation spectra of m/z 771.28 at retention time 15.12 minutes. This oligosaccharide represents #12 in Table 4. c) The fragmentation spectra of m/z 903.32 at retention time 20.41 minutes. This oligosaccharide represents #20 from Table 4. d) The fragmentation spectra of m/z 1227.43 at retention time 26.59 minutes. This oligosaccharide represents #42 from Table 4.

FIG. 7 shows a flowchart outlining strategies for elucidation of oligosaccharide structure.

FIG. 8A shows an annotated chromatogram with completely elucidated oligosaccharides from galactomannan, which were determined using logic strategy 4.

FIG. 8B shows a constructed chromatogram representing the monosaccharide concentration of galactose and mannose in each fraction and the relative glycosidic linkage composition of fractions shown in pie charts.

FIG. 8C shows the NMR analysis of the oligosaccharides.

FIG. 8D shows a representation of the elucidated galactomannan structure by integrating oligosaccharide, monosaccharide, glycosidic linkage, and NMR analysis.

DETAILED DESCRIPTION OF THE INVENTION

Provided herein are novel synthetic oligosaccharides which, when co-formulated with each other, resemble polysaccharides produced by plants, yeast and other fungi, bacteria, and algae. The oligosaccharides can be used for numerous applications including prebiotics, immune modulation, gut health, and analytical standards. The oligosaccharides, which range from 3-20 monosaccharides in length, maintain the same recognizable epitopes that constitute their parent polysaccharides and thus can provide wide ranging bioactivities. The reduction in size enables them to be more easily recognized and internalized by cells.

I. EMBODIMENTS OF THE INVENTION

As used herein, the term “synthetic oligosaccharide” refers to an oligosaccharide produced by the depolymerization of a polysaccharide. Synthetic oligosaccharides according to the present invention can be obtained by depolymerizing heteropolymer polysaccharides and homopolymer polysaccharides according to the methods described herein. As used herein, the term “heteropolymer polysaccharide” refers to a polysaccharide containing two or more kinds of monosaccharide subunits linked together by the same type of glycosidic bond or different types of glycosidic bonds; heteropolymer polysaccharides also include polysaccharides containing repeating monosaccharide subunits of the same kind linked together by different types of glycosidic bonds. The glycosidic bonds in a heteropolymer polysaccharide may be β1-2 bonds, β1-3 bonds, β1-4 bonds, β1-6 bonds, α1-3 bonds, α1-4 bonds, α1-6 bonds, or a combination thereof. Examples of heteropolymer polysaccharides include, but are not limited to, xyloglucan, lichenan, β-glucan, glucomannan, galactomannan, arabinan, xylan, and arabinoxylan.

Some embodiments of the present disclosure provide synthetic oligosaccharides comprising a backbone containing glucose monomers, wherein each glucose monomer is optionally bonded to a pendant xylose monomer, and wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30. Such synthetic oligosaccharides can be obtained, for example, by depolymerizing xyloglucan according to the methods described herein. Xyloglucan is known to contain a glucose backbone with single-unit xylose branches, where the xylose branches may be modified with a galactose endcap or an arabinose endcap. Tamarind xyloglucan, for example, contains a β1,4-linked glucose backbone with frequent single-unit branches of α1,6-linked xylose that can occasionally be further attached to a single β1,2-linked galactose endcap. In other sources of xyloglucan, arabinose can be α1,2 linked to the xylose residue. Xyloglucan from other sources may contain a single fucose residue α1,2 linked to the galactose.

In some embodiments, the oligosaccharides comprise 2, 3, 4, 5, or 6 hexose residues. In some embodiments, the oligosaccharides contain 1, 2, 3, or more pentose residues. In some embodiments, the oligosaccharides contain an equal number of hexose and pentose residues. In some embodiments the oligosaccharides contain fewer pentose residues than hexose residues.

In some embodiments, the glucose monomers in the backbone of the synthetic oligosaccharide are β1-4 linked glucose monomers. In some embodiments, each pendant xylose monomer is bonded to a glucose monomer in the backbone by an α1-6 linkage.

In some embodiments, the synthetic oligosaccharide further includes one galactose monomer bonded to one or more pendant xylose monomers. In some embodiments, each galactose monomer is bonded to the pendant xylose monomer via a β1-2 linkage. In some embodiments, the synthetic oligosaccharide further includes one fucose monomer bonded to one or more galactose monomers. In some embodiments, each fucose monomer is bonded to the galactose monomer via an α1-2 linkage.

In some embodiments, the synthetic oligosaccharide further includes one arabinose monomer bonded to one or more pendant xylose monomers. In some embodiments, the arabinose monomer is bonded to the pendant xylose monomer via an α1-2 linkage.

In some embodiments, the synthetic oligosaccharide contains 2 to 4 glucose monomer in the backbone, 1 to 2 pendant xylose monomers bonded to different glucose monomers in the backbone, and 0 to 2 galactose monomers bonded to different xylose monomers.

In some embodiments, the synthetic oligosaccharide is selected from a compound set forth in Table 4 below. In some embodiments, the synthetic oligosaccharide is selected from a group consisting of any 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, or 34 of the following compounds:

((Galβ1-2)Xylα1-6)Glu,

(Xylα1-6)Gluβ1-4Glu,

Gluβ1-4(Xylα1-6)Glu,

(Xylα1-6)Gluβ1-4(Xylα1-6)Glu,

Gluβ1-4((Galβ1-2)Xylα1-6)Glu,

((Galβ1-2)Xylα1-6)Gluβ1-4Glu,

Gluβ1-4Gluβ1-4(Xylα1-6)Glu,

(Xylα1-6)Gluβ1-4Gluβ1-4Glu,

Gluβ1-4(Xylα1-6)Gluβ1-4Glu,

(Xylα1-6)Gluβ1-4((Galβ1-2)Xylα1-6)Glu,

((Galβ1-2)Xylα1-6)Gluβ1-4(Xylα1-6)Glu,

(Xylα1-6)Gluβ1-4Gluβ1-4(Xylα1-6)Glu,

Gluβ1-4(Xylα1-6)Gluβ1-4(Xylα1-6)Glu,

(Xylα1-6)Gluβ1-4(Xylα1-6)Gluβ1-4Glu,

Gluβ1-4Gluβ1-40Galβ1-2)Xylα1-6)Glu,

Gluβ1-4((Galβ1-2)Xylα1-6)Gluβ1-4Glu,

((Galβ1-2)Xylα1-6)Gluβ1-4Gluβ1-4Glu,

Gluβ1-4Gluβ1-4Gluβ1-4(Xylα1-6)Glu,

Gluβ1-4(Xylα1-6)Gluβ1-4Gluβ1-4Glu,

Gluβ1-4Gluβ1-4(Xylα1-6)Gluβ1-4Glu,

(Xylα1-6)Gluβ1-4Gluβ1-4Gluβ1-4Glu,

(Xylα1-6)Gluβ1-4(Xylα1-6)Gluβ1-4(Xylα1-6)Glu,

((Galβ1-2)Xylα1-6)Gluβ1-4((Galβ1-2)Xylα1-6)Glu,

Gluβ1-4(Xylα1-6)Gluβ1-4((Galβ1-2)Xylα1-6)Glu,

Gluβ1-4((Galβ1-2)Xylα1-6)Gluβ1-4(Xylα1-6)Glu,

(Xylα1-6)Gluβ1-4Gluβ1-4((Galβ1-2)Xylα1-6)Glu,

((Galβ1-2)Xylα1-6)Gluβ1-4Gluβ1-4(Xylα1-6)Glu,

((Galβ1-2)Xylα1-6)Gluβ1-4(Xylα1-6)Gluβ1-4Glu,

(Xylα1-6)Gluβ1-4((Galβ1-2)Xylα1-6)Gluβ1-4Glu,

Gluβ1-4Gluβ1-4(Xylα1-6)Gluβ1-4(Xylα1-6)Glu,

Gluβ1-4(Xylα1-6)Gluβ1-4(Xylα1-6)Gluβ1-4Glu,

(Xylα1-6)Gluβ1-4Gluβ1-4(Xylα1-6)Gluβ1-4Glu,

Gluβ1-4(Xylα1-6)Gluβ1-4Gluβ1-4(Xylα1-6)Glu, and

(Xylα1-6)Gluβ1-4Gluβ1-4Gluβ1-4(Xylα1-6)Glu.

Some embodiments of the present disclosure provide synthetic oligosaccharides having a backbone containing mannose monomers, wherein each mannose monomer is optionally bonded to a pendant galactose monomer, and wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30. Such synthetic oligosaccharides can be obtained, for example, by depolymerizing galactomannan according to the methods described herein. Galactomannan, produced by sources such as Aspergillus molds, contains a β1-4 mannose backbone with frequent α1-6 galactose branches containing a single unit (34).

In some embodiments, the synthetic oligosaccharide is selected from a compound set forth in Table 5-9 below. In some embodiments, the synthetic oligosaccharide is selected from a group consisting of any 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 of the following compounds:

Manβ1-4Manβ1-4Man,

(Galα1-6)Manβ1-4Man,

Manβ1-4(Galα1-6)Man,

Manβ1-4Manβ1-4Manβ1-4Man,

Manβ1-4(Galα1-6)Manβ1-4Man,

(Galα1-6)Manβ1-4Manβ1-4Man,

Manβ1-4Manβ1-4(Galα1-6)Man,

(Galα1-6)Manβ1-4(Galα1-6)Man,

Manβ1-4[Manβ1-4]₃Man,

(Galα1-6)Manβ1-4Manβ1-4Manβ1-4Man,

Manβ1-4(Galα1-6)Manβ1-4Manβ1-4Man,

Manβ1-4Manβ1-4(Galα1-6)Manβ1-4Man,

Manβ1-4Manβ1-4Manβ1-4(Galα1-6)Man,

(Galα1-6)Manβ1-4Manβ1-4(Galα1-6)Man,

(Galα1-6)Manβ1-4(Galα1-6)Manβ1-4Man,

Manβ1-4[Manβ1-4]₄Man,

(Galα1-6)Manβ1-4Manβ1-4Manβ1-4Manβ1-4Man,

Manβ1-4(Galα1-6)Manβ1-4Manβ1-4Manβ1-4Man,

Manβ1-4Manβ1-4(Galα1-6)Manβ1-4Manβ1-4Man,

Manβ1-4Manβ1-4Manβ1-4(Galα1-6)Manβ1-4Man,

Manβ1-4Manβ1-4Manβ1-4Manβ1-4(Galα1-6)Man,

(Galα1-6)Manβ1-4Manβ1-4Manβ1-4Manβ1-4Manβ1-4Man,

Manβ1-4(Galα1-6)Manβ1-4Manβ1-4Manβ1-4Manβ1-4Man,

Manβ1-4Manβ1-4(Galα1-6)Manβ1-4Manβ1-4Manβ1-4Man,

Manβ1-4Manβ1-4Manβ1-4(Galα1-6)Manβ1-4Manβ1-4Man,

Manβ1-4Manβ1-4Manβ1-4Manβ1-4(Galα1-6)Manβ1-4Man,

Manβ1-4Manβ1-4Manβ1-4Manβ1-4Manβ1-4(Galα1-6)Man

Manβ1-4[Manβ1-4]₅Man,

Manβ1-4[Manβ1-4]₆Man, and

Manβ1-4[Manβ1-4]₇Man.

Some embodiments of the present disclosure provide synthetic oligosaccharides containing mannose monomers and glucose monomers, wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30. Such synthetic oligosaccharides can be obtained, for example, by depolymerizing glucomannan according to the methods described herein. Glucomannan is a polysaccharide largely known to be found in konjac root. The polymer contains β1-4-linked glucose and mannose residues that are thought to be randomly distributed in a non-reoccurring pattern (38).

In some embodiments, the synthetic oligosaccharide is selected from a compound set forth in Table 5-8 below. In some embodiments, the synthetic oligosaccharide is selected from a group consisting of any 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 of the following compounds:

Manβ1-4Manβ1-4Man,

Glcβ1-4Manβ1-4Man,

Manβ1-4Glcβ1-4Man,

Manβ1-4Manβ1-4Glc,

Glcβ1-4Glcβ1-4Man,

Glcβ1-4Manβ1-4Glc,

Manβ1-4Glcβ1-4Glc,

Manβ1-4Manβ1-4Manβ1-4Man,

Glcβ1-4Manβ1-4Manβ1-4Man,

Glcβ1-4Glcβ1-4Manβ1-4Man,

Glcβ1-4Manβ1-4Glcβ1-4Man,

Glcβ1-4Manβ1-4Manβ1-4Glc,

Glcβ1-4Glcβ1-4Glcβ1-4Man,

Glcβ1-4Glcβ1-4Manβ1-4Glc,

Glcβ1-4Manβ1-4Glcβ1-4Glc,

Manβ1-4Glcβ1-4Manβ1-4Man,

Manβ1-4Manβ1-4Glcβ1-4Man,

Manβ1-4Manβ1-4Manβ1-4Glc,

Manβ1-4Glcβ1-4Glcβ1-4Man,

Manβ1-4Glcβ1-4Manβ1-4Glc,

Manβ1-4Manβ1-4Glcβ1-4Glc,

Manβ1-4Glcβ1-4Glcβ1-4Glc,

Manβ1-4[Manβ1-4]₃Man,

Manβ1-4[Manβ1-4]₄Man, and

Manβ1-4[Manβ1-4]₅Man.

Some embodiments of the present disclosure provide synthetic oligosaccharides having a backbone containing arabinose monomers, wherein each arabinose monomer is optionally bonded to a pendant arabinose monomer, and wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30. Such synthetic oligosaccharides can be obtained, for example, by depolymerizing arabinan according to the methods described herein. Arabinans exist as sidechains on the pectin polysaccharide rhamnogalacturonan I and also in the cell walls of some mycobacteria (37). Arabinan contains an α1-5 arabinose backbone with short α1-3 arabinose branches.

In some embodiments, the synthetic oligosaccharide is selected from a compound set forth in Table 5-12 below. In some embodiments, the synthetic oligosaccharide is selected from a group consisting of any 1, 2, 3, 4, 5, 6, or 7 of the following compounds:

Araα1-5Araα1-5Ara,

(Araα1-3)Araα1-5Ara,

Araα1-5(Araα1-3)Ara,

Araα1-5Araα1-5Araα1-5Ara,

(Araα1-3)Araα1-5Ara1-5Ara,

Araα1-5(Araα1-3)Ara1-5Ara, and

Araα1-5Ara1-5(Araα1-3)Ara.

β-Glucans found in cereals (e.g., rice, wheat, oat, bran, barley, and malt), for example, consist of a β1-4 linked glucose backbone with single β1-3 glucose residues dispersed between every 2-3 β1-4 linked glucose residues (31). Lichenan is a polysaccharide found in lichen, having a structure is similar to β-glucan where the linkages consist of β1-4 and β1-3 glucose residues (32). However, unlike β-glucan, lichenan has much more frequent β1-3 linkages.

In some embodiments, the synthetic oligosaccharide is selected from a compound set forth in Table 5-6 or Table 5-7 below. In some embodiments, the synthetic oligosaccharide is selected from a group consisting of any 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, or 17 of the following compounds:

Glcβ1-4Glcβ1-3Glc,

Glcβ1-3Glcβ1-4Glc,

Glcβ1-3Glcβ1-4Glc1-4Glc,

Glcβ1-4Glcβ1-3Glc1-4Glc,

Glcβ1-4Glcβ1-4Glc1-3Glc,

Glcβ1-4Glcβ1-4Glc1-3Glc

Glcβ1-3Glcβ1-4Glc1-3Glc,

Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc,

Glcβ1-4Glcβ1-3Glc1-4Glc1-4Glc,

Glcβ1-4Glcβ1-4Glc1-3Glc1-4Glc,

Glcβ1-4Glcβ1-4Glc1-4Glc1-3Glc,

Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc1-4Glc,

Glcβ1-4Glcβ1-3Glc1-4Glc1-4Glc1-4Glc,

Glcβ1-4Glcβ1-4Glc1-3Glc1-4Glc1-4Glc,

Glcβ1-4Glcβ1-4Glc1-4Glc1-3Glc1-4Glc,

Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc1-3Glc, and

Glcβ1-4Glcβ1-4Glc1-4Glc1-4Glc1-3Glc.

Some embodiments of the present disclosure provide synthetic oligosaccharides having a backbone containing xylose monomers, wherein each xylose monomer is optionally bonded to a pendant arabinose monomer or a pendant gluronic acid (e.g., a 4-0 methylated GlcA), and wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30. Such synthetic oligosaccharides can be obtained, for example, by depolymerizing xylan and/or arabinoxylan according to the methods described herein. Xylan is a polysaccharide commonly found in the secondary cell walls of dicots and in the cell walls of most grasses. The structure contains a β1-4 xylose backbone and often times contains α1-2 glucuronic acid branches, which may contain a single methyl group. In this experiment, beechwood xylan was used which is known to contain large amounts of 4-O-methyl-glucuronic acid branches (35). Arabinoxylan is a polysaccharide commonly found in cereals grains that contains a β1-4 xylose backbone with α1-2 and α1-3 arabinose branches (36).

In some embodiments, the synthetic oligosaccharide is selected from a compound set forth in Table 5-10 below. In some embodiments, the synthetic oligosaccharide is selected from a group consisting of any 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 of the following compounds:

Xylβ1-4Xylβ1-4Xyl,

Xylβ1-4Xylβ1-4Xylβ1-4Xyl,

(OMe-4-GlcAα1-2)Xylβ1-4Xylβ1-4Xyl,

Xylβ1-4(OMe-4-GlcAα1-2)Xylβ1-4Xyl,

Xylβ1-4Xylβ1-4(OMe-4-GlcAα1-2)Xyl,

Xylβ1-4[Xylβ1-4]₃Xyl,

(OMe-4-GlcAα1-2)Xylβ1-4Xylβ1-4Xylβ1-4Xyl,

Xylβ1-4(OMe-4-GlcAα1-2)Xylβ1-4Xylβ1-4Xyl,

Xylβ1-4Xylβ1-4(OMe-4-GlcAα1-2)Xylβ1-4Xyl,

Xylβ1-4Xylβ1-4Xylβ1-4(OMe-4-GlcAα1-2)Xyl,

Xylβ1-4[Xylβ1-4]₄Xyl,

(OMe-4-GlcAα1-2)Xylβ1-4[Xylβ1-4]₃Xyl,

Xylβ1-4(OMe-4-GlcAα1-2)Xylβ1-4[Xylβ1-4]₂Xyl,

Xylβ1-4Xylβ1-4(OMe-4-GlcAα1-2)Xylβ1-4Xylβ1-4Xyl,

[Xylβ1-4]₃(OMe-4-GlcAα1-2)Xylβ1-4Xyl,

[Xylβ1-4]₄(OMe-4-GlcAα1-2)Xyl,

Xylβ1-4[Xylβ1-4]5Xyl,

(OMe-4-GlcAα1-2)Xylβ1-4 [Xylβ1-4]₄Xyl,

Xylβ1-4(OMe-4-GlcAα1-2)Xylβ1-4[Xylβ1-4]₃Xyl,

Xylβ1-4Xylβ1-4(OMe-4-GlcAα1-2)Xylβ1-4[Xylβ1-4]₂Xyl,

[Xylβ1-4]3(OMe-4-GlcAα1-2)Xylβ1-4Xylβ1-4Xyl,

[Xylβ1-4]₄(OMe-4-GlcAα1-2)Xylβ1-4Xyl,

[Xylβ1-4]₅(OMe-4-GlcAα1-2)Xyl, and

Xylβ1-4[Xylβ1-4]₆Xyl.

In some embodiments, the synthetic oligosaccharide is selected from a compound set forth in Table 5-11 below. In some embodiments, the synthetic oligosaccharide is selected from a group consisting of any 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 of the following compounds:

(Araα1-3)Xylβ1-4Xyl,

Xylβ1-4(Araα1-3)Xyl,

((Araα1-2)(Araα1-3))Xyl,

Xylβ1-4Xylβ1-4Xyl,

(Araα1-3)Xylβ1-4Xylβ1-4Xyl,

Xylβ1-4(Araα1-3)Xylβ1-4Xyl,

Xylβ1-4Xylβ1-4(Araα1-3)Xyl,

(Araα1-3)Xylβ1-4(Araα1-3)Xyl,

((Araα1-2)(Araα1-3))Xylβ1-4Xyl,

Xylβ1-4((Araα1-2)(Araα1-3))Xyl,

Xylβ1-4Xylβ1-4Xylβ1-4Xyl,

Xylβ1-4[Xylβ1-4]3Xyl,

Xylβ1-4[Xylβ1-4]4Xyl,

Xylβ1-4[Xylβ1-4]5Xyl, and

Xylβ1-4[Xylβ1-4]6Xyl.

Synthetic oligosaccharides can be also be obtained by depolymerizing homopolymer polysaccharides according to the methods described herein. As used herein, the term “homopolymer polysaccharide” refers to a polysaccharide containing repeating monosaccharide subunits of the same kind, linked together by the same type of glycosidic bond including, but not limited to, a combination of β1-3 bonds, β1-4 bonds, β1-6 bonds, al-3 bonds, α1-4 bonds, and α1-6 bonds. Examples of homo polymers include, but are not limited to, curdlan, galactan, and mannan. Homopolymers include, but are not limited to, curdlan (a linear polymer of β1-3 linked glucose found as an exopolysaccharide of Agrobacterium; 28), galactan (a linear polymer of β1-4 linked galactose that has been isolated in the form of arabinogalactan before subsequent arabinofuranosidase treatment to remove the arabinose units; 29), and mannan (a linear polymer of β1-3 linked glucose found as an exopolysaccharide of Agrobacterium and also some nuts; 30).

In some embodiments, the synthetic oligosaccharide is selected from a compound set forth in Table 5-3, Table 5-4, and Table 5-5 below. In some embodiments, the synthetic oligosaccharide is selected from a group consisting of any 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, or 18 of the following compounds:

Glcβ1-3Glcβ1-3Glc,

Glcβ1-3Glcβ1-3Glcβ1-3Glc,

Glcβ1-3[Glcβ1-3]₃Glc,

Glcβ1-3[Glcβ1-3]₄Glc,

Galβ1-4Galβ1-4Gal,

Galβ1-4Galβ1-4Galβ1-4Gal,

Galβ1-4[Galβ1-4]₃Gal,

Galβ1-4[Galβ1-4]₄Gal,

Galβ1-4[Galβ1-4]₅Gal,

Galβ1-4[Galβ1-4]₆Gal,

Galβ1-4[Galβ1-4]₇Gal,

Manβ1-4Manβ1-4Man,

Manβ1-4Manβ1-4Manβ1-4Man,

Manβ1-4[Manβ1-4]₃Man,

Manβ1-4[Manβ1-4]₄Man,

Manβ1-4[Manβ1-4]₅Man,

Manβ1-4[Manβ1-4]₆Man, and

Manβ1-4[Manβ1-4]₇Man.

The synthetic oligosaccharides can be prepared by any suitable method including, but not limited to, Fenton's Initiation Toward Defined Oligosaccharide Groups (FITDOG) which is a method for the controlled degradation of polysaccharides into oligosaccharides. Exemplary FITDOG procedures are described, for example, in US Pat. Appl. Pub. No. 2018/0363016 A1, which is incorporated herein by reference in its entirety. In some embodiments, the crude polysaccharides first undergo initial oxidative treatment with the hydrogen peroxide and a transition metal or alkaline earth metal (e.g., iron(III) sulfate) catalyst to render the glycosidic linkages more labile. NaOH or other base is then used for base induced cleavage, which results in a variety of oligosaccharides. Immediate neutralization takes place to reduce the peeling reaction. This method has the ability to generate large amounts of biologically active oligosaccharides from a variety of carbohydrate sources.

If desired, the polysaccharide can be optionally treated with one or more polysaccharide-degrading enzyme to reduce the average size or complexity of the polysaccharide before the resulting polysaccharides are treated with the oxidative treatment and metal catalyst. Non-limiting examples of polysaccharide enzymes include for example, amylase, isoamylase, cellulase, maltase, glucanase, or a combination thereof.

The initial oxidative treatment can include hydrogen peroxide and a transition metal or an alkaline earth metal. Metals with different oxidation states, sizes, periodic groups, and coordination numbers have been tested to understand the application with the FITDOG process. Each of the different metals has shown activity in the FITDOG reaction. While these metals work with any polysaccharide, different metals can be used to produce oligosaccharides with preferential degrees of polymerization. The oxidative treatment is followed by a base treatment. The method is capable of generating oligosaccharides from polysaccharides having varying degrees of branching, and having a variety of monosaccharide compositions, including natural and modified polysaccharides.

Also provided are mixtures containing two or more different synthetic oligosaccharides as described herein. Unpurified or semi-purified depolymerization products may be used for preparation of oligosaccharide mixtures or, alternatively, oligosaccharides can be purified to produce specially formulated pools. The synthetic oligosaccharides in the mixtures may be obtained, for example, by depolymerizing a polysaccharide homopolymer, a polysaccharide heteropolymer or a combination thereof. In some embodiments, at least one of the synthetic oligosaccharides in the mixture is obtained via depolymerization of xyloglucan, curdlan, galactan, mannan, lichenan, β-glucan, glucomannan, galactomannan, arabinan, xylan, arabinoxylan, or a combination thereof. The compositions may include any number of synthetic oligosaccharides as set forth in Tables 4-1, 5-3, 5-4, 5-5, 5-6, 5-7, 5-8, 5-9, 5-10, 5-11, and 5-12. In some embodiments, the amount of at least one of the synthetic oligosaccharides in the mixture is at least 1%, based on the total amount of oligosaccharides in the mixture. The synthetic oligosaccharide may be present, for example, in an amount ranging from about 1% to about 99%, or from about 5% to about 95%, or from about 10% to about 90%, or from about 20% to about 80%, or from about 30% to about 70%. The synthetic oligosaccharide may be present, for example, in an amount ranging from about 1% to about 10%, or from about 10% to about 20%, or from about 20% to about 30%, or from about 30% to about 40%, or from about 40% to about 50%, or from about 50% to about 60%, or from about 60% to about 70%, or from about 70% to about 80%, or from about 80% to about 90%, or from about 90% to about 99%. The percentage may be a mol %, based on the total number of moles of oligosaccharides in the mixture, or a weight %, based on the total weight of oligosaccharides in the mixture. In some embodiments, the amount of at least one of synthetic oligosaccharides is at least 5 mol %.

As used herein, the terms “about” and “around” indicate a close range around a numerical value when used to modify that specific value. If “X” were the value, for example, “about X” or “around X” would indicate a value from 0.9X to 1.1X, e.g., a value from 0.95X to 1.05X, or a value from 0.98X to 1.02X, or a value from 0.99X to 1.01X. Any reference to “about X” or “around X” specifically indicates at least the values X, 0.9X, 0.91X, 0.92X, 0.93X, 0.94X, 0.95X, 0.96X, 0.97X, 0.98X, 0.99X, 1.01X, 1.02X, 1.03X, 1.04X, 1.05X, 1.06X, 1.07X, 1.08X, 1.09X, and 1.1X, and values within this range.

The synthetic oligosaccharides and compositions described herein are useful as synbiotics, prebiotics, immune modulators, digestion aids, food additives, pharmaceutical excipients, or analytical standards. The synthetic oligosaccharides can be combined with other ingredients to produce foodstuffs and supplements including infant formula, geriatric supplements, baking flours, and snack foods. The synthetic oligosaccharides can be combined with beneficial bacteria to form synbiotics. The synthetic oligosaccharides can also be used as pharmaceutical products.

The synthetic oligosaccharides can be used as for growth or maintenance of specific microorganism in humans, other mammals, or in the rhizosphere of plants. The synthetic oligosaccharides may contain specific glycosidic linkages not able to be digested by the particular host (e.g., a person, livestock animal, or companion animal) but able to be metabolized by specific groups of commensal microorganism or probiotics. As such, the synthetic oligosaccharides can function as a carrier to transport exogenous microorganisms (probiotic or bio therapeutic) to a specific niche, or as a nutritional source for microorganisms already present in the host.

Xyloglucan can be used for the selective growth of specific Bacteroides species, like B. ovatus (39). It has been demonstrated that the xyloglycan utilization loci, with glycoside hydrolase genes, belongs to the families GH5 and GH31 which can be found in B. ovatus. The presence of these genes allow the growth of this species when used as a sole carbon source. Other major Bacteroides species in the gut like B. thetaiotaomicron, B. caccae or B. fragilis, lack this loci or part of it in their genomes, and, thusly, are unable to metabolize xyloglucan.

Curdlan can be used for the selective growth of specific Bacteroides species, like B. thetaiotaomicron or B. distasonis, when their genomes encode a specific type of glycoside hydrolase belonging to the family GH16. Orthologs of this gene are absent in the genomes of other Bacteroides species like B. caccae or B. ovatus, and are unable to grow on curdlan (40).

β-glucan or lichenin can be used for the selective growth of specific Bacteroides species, like B. ovatus. This species encodes in its genome a specific type of GH16, with β1-3,4 glucan activity (41). It has been demonstrated that this polysaccharides enhances the growth of species of Firmicutes like Enterococcus faecium, Clostridium perfingens, Roseburia inulinivorans, and R. faecis (42, 43).

Galactan can select for the growth of specific Bacteroides species, such as B. thetaiotaomicron, B. dorei and B. ovatus. Different types of endo-galactanases can be responsible for this selective growth, which belong to the families GH53 and GH147 (44; 45). The ability to consume galactan has also been described in some Bifidobacterial species (Bif. breve, Bif. longum, Bif. long subsp. Infantis) (46)

Mannan can selectively growth specific Bacteroides species, like B. fragilis or B. ovatus, which encode a GH26 endo-β 1-4-mannosidase (48). This gene is absent in the genome of major intestinal species like B. thetaiotamicron, which are unable to grow on mannan or glucomannan. R. intestinalis and R. faecis can deplete mannan linkages (49), as well as members of Clostridium cluster XIVa (47, 43), with GH26 encoded in their genomes. Also, GH26 has been characterized in specific species of Bifidobacteria, such as Bif. adolescentis (50), confirming the ability of this species to grow on mannan. Galactomannan is consumed only by microorganism that encode endo-β 1-4-mannosidase GH26 and alpha-galactosidase GH27 in their genomes, like B. ovatus, B. xylanisolvens (51) or Roseburia intestinalis (47, 49).

Xylan, arabinan and arabinoxylan can be used to selectively grow specific species of Bacteroides. Xylan can be metabolized by B. ovatus and B. uniformis, while B. thetaiotaomicron or B. caccae are unable to grow in this substrate. Arabinan promotes the growth of B. thetaiotaomicron and B. ovatus, while arabinoxylan shows high selection for B. ovatus growth (52, 47). It has been shown that strains of R. intestinalis, E. rectale and R. faecis can consume xylan or arabinoxylan as the sole carbon source (47, 43). Certain bifidobacteria have the capacity to ferment xylan or arabinofuranosyl-containing oligosaccharides. Selective growth of B. adolescentis on xylose and arabinoxylan derived glycans was shown in vitro (53). Also, additional experiment confirmed that B. longum subsp. longum was also able to metabolize arabinoxylan (54)

II. EXAMPLES Example 1. Materials and Methods

Experimental Materials. Sodium acetate, hydrogen peroxide (H₂O₂), sodium hydroxide (NaOH), sodium borohydride (NaBH₄), iron(III) sulfate pentahydrate (Fe₂(SO₄)₃), and glacial acetic acid were purchased from Sigma-Aldrich (St. Louis, Mo.). Amylopectin was obtained from Carbosynth (Compton, UK). Amylose, xyloglucan, arabinoxylan, xylan, glucomannan, galactomannan, mannan, curdlan, galactan, β-glucan, arabinan, and lichenan were purchased from Megazyme (Bray, Ireland). Microcrystalline cellulose was purchased from ACROS Organics. Formic Acid (FA) was purchased from Fisher Scientific (Belgium, UK). Acetonitrile (HPLC grade) was purchased from Honeywell (Muskegon, Mich.). Nanopure water was used for all experiments.

Generation of Oligosaccharides via Fenton's Initiation Towards Defined Oligosaccharide Groups (FITDOG). A solution was prepared containing 95% (v/v) 40 mM sodium acetate buffer adjusted to pH 5 with glacial acetic acid, 5% (v/v) hydrogen peroxide (30% v/v), and 65 nM iron(III) sulfate. This mixture was vortexed and added to dry polysaccharide standards to make a final solution of 1 mg/ml. The reaction was incubated at 100° C. for one hour. The reaction was quenched by adding half of the reaction volume of cold 2 M NaOH. Glacial acetic acid was added for neutralization.

Oligosaccharides were reduced by incubation with 1 M NaBH₄ for one hour at 65° C. Oligosaccharides were isolated using nonporous graphitized carbon cartridges. Cartridges were washed with 80% acetonitrile and 0.1% (v/v) TFA in water. The oligosaccharides were loaded and washed with five column volumes of water. The oligosaccharides were eluted with 40% acetonitrile with 0.05% (v/v) TFA. Samples were completely dried by evaporative centrifugation and stored at −20° C. until analysis.

Sample Preparation for Fecal Fingerprinting. To separate the endogenous oligosaccharides from the polysaccharides, pear and feces samples underwent 80% ethanol precipitation overnight at −80° C. Samples were centrifuged at 3000 rpm for 20 minutes to pellet the polysaccharide fraction and partition it from the endogenous oligosaccharides in the supernatent. The pelletedfraction underwent FITDOG treatment to generate representative oligosaccharides. Oligosaccharides from the FITDOG treatment were reduced and purified using the protocol described in the section above.

MALDI MS Analysis. For analysis by MALDI-MS, 1 μl was plated directly onto a stainless steel MALDI plate. To this, 0.3 μl of 0.01M NaCl and 0.7 μl of 25 mg/ml 2, 5-dihydroxybenzoic acid was added and mixed within the pipet tip. The samples were dried under vacuum and analyzed on a Bruker UltraFlextreme MALDI-tandem time-of-flight (MALDI-TOF/TOF) instrument. The instrument was operated in positive mode and 95% of max laser power.

Nano-HPLC-chip/Q-TOF Analysis. Samples were reconstituted in nano-pure water before analysis by nano-HPLC-chip/Q-TOF MS. The system includes two pumps, a capillary pump for sample loading and a nano pump for analytical separation. In this system, an Agilent 1200 series HPLC is coupled to an Agilent 6520 Q-TOF mass spectrometer through a chip cube interface. The chip contains a 40 nl enrichment column, and a 75 μm×43 mm analytical column; both columns have PGC as the stationary phase. Sample loading was done with 3% (v/v) acetonitrile/water+0.1% formic acid at a flow rate of 4 μl/min. Chromatographic separation was performed with a binary gradient of solvent A: (3% (v/v) acetonitrile/water+0.1% formic acid) and solvent B: (90% acetonitrile/water+0.1% formic acid) with a flow rate of 0.4 μl/min. The gradient was run for 60 minutes, 1-5% B, 0-2 min; 5-30%, 2-33 min; 30-99%, 33-38 min; 99-99%, 38-48 min; 99-1%, 48-50 min; 1-1%, 50-60 min before starting the next run.

Data were collected in the positive mode and calibrated with internal calibrant ions ranging from m/z 118.086 to 2721.895. Drying gas was set to 325° C. and with a flow rate of 5 l/min. The fragment, skimmer, and Octapole 1 RF voltages were set to 175, 60, and 750 V, respectively. Fragmentation was performed at a rate of 0.63 spectra/second. The collision energy was based upon the compound mass and expressed by the linear function (Collision Energy)=1.8*(m/z)-2.4.

HPLC Q-TOF Analysis. Samples were reconstituted in nano-pure water before HPLC Q-TOF MS analysis. The analytical separation was carried out using an Agilent 1260 Infinity II HPLC coupled to an Agilent 6530 Accurate-Mass Q-TOF MS. Chromatographic separation was performed on a 150 mm×1 mm Hypercarb column from Thermo Scientific with a 5 μm particle size. A binary gradient was employed which consisted of solvent A: (3% (v/v) acetonitrile/water+0.1% formic acid) and solvent B: (90% acetonitrile/water+0.1% formic acid). A 45 min gradient with a flow rate of 0.150 mL/min was used for chromatographic separation: 3-25% B, 0-15 min; 25-25% B, 15-18 min; 25-99% B, 18-30 min; 99-99% B, 30-32 min; 99-3% B, 32-34 min; 3-3% B, 34-45 min. Samples were run in the positive mode. Internal calibrant ions ranged from m/z 121.051 to m/z 2421.914. Drying gas temperature and flow rate were set to 150° C. and 11 l/min, respectively. Operation voltages for the fragment, skimmer, and octupole 1 RF were 175, 60, and 750 V, respectively. The acquisition rate was set to 0.63 spectra/second. When fragmentation was used, the linear function, Collision Energy=1.45*(m/z)−3.5, was employed.

Infant Fecal Samples. The fecal sample was collected from a healthy, term 6-month-old infant who completed the UC Davis Infant Microbiome Nutrition and Development (Infant MiND) Study. The infant was exclusively breastfed at enrollment. The infant was assigned to consume pear (Earth's Best Stage 1) concurrently with breast milk for seven days. After seven days, parents were instructed to scrape the soiled diaper with sterile utensils, to place the fecal samples into sterile tubes, and to seal and store the samples in their kitchen freezers. The fecal samples were transported back to University of California Davis campus on dry ice and stored in −80° C. before being analyzed. The University of California Davis Institutional Review Board approved all aspects of this study and written informed consent was obtained from the participants. This study was registered on clinicaltrials.gov (NCT01817127).

Monosaccharide Analysis. Monosaccharides were analyzed in the manner of Amicucci et al.²⁴ Briefly, the oligosaccharides were hydrolyzed using trifluoroacetic acid (TFA) and derivatized with 3-methyl-1-phenyl-pyrazoline-5-one (PMP). The derivatized oligosaccharides were extracted twice with chloroform and water and analyzed by UHPLC/QqQ MS as described by Xu et al.²⁵

Glycosidic Linkage Analysis. Glycosidic linkages were analyzed in the manner of Galermo et al.²⁶ Briefly, the oligosaccharides were first permethylated with iodomethane in the presence of sodium hydroxide and dimethylsulfoxide. The permethylated oligosaccharides were extracted with water and DCM to remove the dimethylsulfoxide. Next, the oligosaccharides were hydrolyzed with TFA and derivatized with PMP. The partially permethylated monosaccharides were then analyzed by UHPLC-QqQ MS under the same conditions described by Galermo et al.²⁶

Example 2. Production of Oligosaccharides by FITDOG

A polysaccharide with a known structure, xyloglucan, was used to determine the characteristics of the method. Xyloglucan was comprised of a β(1>4) backbone with frequent β(1>6) xylose branches that were often terminated with a single β(1>2) galactose residue.^(13,30-32) The reaction of xyloglucan yielded over 20 structurally unique oligosaccharides as determined by nano-HPLC-chip/Q-TOF MS. The most abundant oligosaccharides are shown in FIG. 1A.

The nanoLC-MS profile of xyloglucan oligosaccharides ranged from disaccharides to hexasaccharides. To obtain the chromatogram, the product mixture was first reduced using sodium borohydride to yield an alditol if a reducing sugar were present. The mixture was enriched and purified as described in the Methods Section. The accurate mass analysis and tandem MS of the products indicated that the reaction produced primarily intact oligosaccharide species. The fragmentation mass spectra obtained through CID were interpreted and yielded the inset structures in FIG. 1A. A combination of oligosaccharides with several degrees of polymerization (DP) were present in the chromatograms. The relatively small numbers of oligosaccharides indicated a degree of specificity in the FITDOG reaction.

While DP can be obtained from the accurate masses, structural information requires tandem MS. Isomers, which have the same DP but different structures, were also present for several DP and were elucidated as follows. Four isomers with the composition, three hexoses, two pentoses (m/z 771.27) were observed at 16.0, 18.9, 21.0, and 31.2 minutes, respectively (FIG. 1B). These isomers were distinguishable by their MS/MS spectra (FIG. 1C). The compounds eluting at 16.0 (I) and 18.9 (II) minutes were determined to contain a 2Hex backbone, due to the lack of a peak representing three hexoses with a reducing monosaccharide (m/z 507.19), as well as the presence of a peak representing two hexoses without a reducing monosaccharide (m/z 325.11). Both isomers contained the peak representing two hexoses, two pentoses, and a reducing end (m/z 609.22), but no peak were present representing two pentoses without a reducing end (m/z 265.09). This suggested that there is one pentose branching from each hexose. Because the backbone contained only two hexoses, the remaining hexose must have been extended from one of the pentoses. Isomer (I) showed a peak representing two hexoses, one pentose, with no reducing end (m/z 457.15), which localized the remaining hexose to the pentose that is not located on the reducing end hexose. Isomer (II) lacks the fragment with m/z 457.15, which localized the remaining hexose to the pentose connected to the reducing end hexose. The final two isomers at 21.0 (III) and 31.2 (IV) minutes both showed a strong peak corresponding to three hexoses with one reducing end (m/z 507.19), which suggested that all three hexoses make up the oligosaccharide backbone. Both isomers also showed an abundant peak representing one hexose, one pentose, and a reducing end (m/z 315.13), which provided evidence of one pentose branching from the reducing end. Finally, the remaining pentose must be branched from one of the two non-reducing end hexoses. Isomer (III) showed a peak that corresponded to two hexoses, two pentoses, and a reducing end (m/z 609.22), which suggested that the second pentose was localized on the hexose adjacent to the reducing end hexose. Isomer (IV) lacked the fragment m/z 609.22 and thus, the second pentose was assigned to the hexose farthest from the reducing end. This rigorous approach was employed to characterize all of the oligosaccharides shown in this report. In accordance with the known structure of xyloglucan, the derived oligosaccharides were found to be primarily composed of hexose backbones with frequent pentose branches that were occasionally terminated by a hexose.

Other polysaccharide standards were also subjected to FITDOG and all yielded representative oligosaccharides (FIGS. 2A-2N). The polysaccharide standards were composed of many monosaccharide combinations, linkage types, and branching patterns. Some contained monosaccharide modifications. The standards included amylose, amylopectin, cellulose, curdlan, lichenan, β-glucan, arabinan, xylan, arabinoxylan, mannan, galactomannan, glucomannan, galactan, and xyloglucan. Annotated chromatograms for each polysaccharide after treatment with FITDOG are shown in FIGS. 2A-2N. Prominent peaks were labeled with the DP and an abbreviation of their monosaccharide components (Hexose/Hex, Pentose/Pent, Hexuronic Acid/HexA).

Among the simplest group of polysaccharides were homopolysaccharides, which are composed of a single monosaccharide and glycosidic linkage. The resulting oligosaccharides from these polysaccharides are expected and were observed to have one structure per DP. For example, curdlan, a linear β(1→3) linked glucose polymer yielded oligosaccharides with compositions corresponding to 3Hex (RT 12.56 min), 4Hex (19.28 min), and 5Hex (26.44 min) (FIG. 2A). Other homopolysaccharides, such as cellulose and amylose also produced one oligomer per DP indicative of their uniform monosaccharide and linkage composition (FIGS. 2B and 2C). Similar trends were reflected in both galactan, a β(1→4) linked galactose polysaccharide, and mannan, a β(1→4) linked mannose polysaccharide. However, some inherent impurities were observed as a result of the processing steps for polysaccharide isolation by the manufacturer (FIGS. 2D and 2E).

Linear heteropolysaccharides contain more than one monosaccharide or glycosidic linkage. The structural heterogeneity contributed to the production of several oligosaccharide isomers for each DP. Among the group, both β-glucan and lichenan were similar in structure and comprise of β(1→3) and β(1→4) linked glucose residues, but in differing ratios. Indeed, LC-MS analysis yielded several isomers associated with each DP. Thus, the composition corresponding to 6Hex was observed to generate eight isomers from lichenan and four isomers from β-glucan (FIGS. 2F and 2G). Additionally, the structural similarities in monosaccharide and linkage composition between lichenan and β-glucan yielded many similar oligosaccharides from both polysaccharides. For instance, the 3Hex (13.73 min), 4Hex (19.46, 19.70, 20.41 min), 5Hex (24.92, 25.79 min), and 6Hex (30.90 min) are potentially similar structures shared between lichenan and β-glucan (FIGS. 2F and 2G). Likewise, glucomannan, another linear heteropolymer containing β(1→4) linked glucose and mannose residues, produced several isomers for each DP (FIG. 2H).

Branched heteropolysaccharides contained an additional layer of complexity that originated from the frequency of branching and/or variations in the glycosidic linkages and monosaccharide constituents. As expected, variations in the positions and frequency of the branches produced many isomers for each DP. For example, galactomannan contained a β(1→4) linked mannose backbone with terminal galactose branches which produced three 3Hex isomers. In sections of the polysaccharide backbone where there was a lack of galactose branching, the monosaccharide arrangement resembled that of mannan. By comparing the oligosaccharides generated from structurally similar polysaccharides, we inferred additional information regarding their oligosaccharide structures. Therefore, the three isomers of 3Hex could be produced from either the mannose backbone or from the mannose backbone with galactose branches. We determined that the 3Hex that eluted at 1.51 min was comprised of three β(1→4) linked mannose residues because it was produced from both sources (FIG. 2I). By deduction, we concluded that the 3Hex oligosaccharides eluting at 1.78 min and 2.55 min originated from two β(1→4) linked mannose constituents with a galactose branch (FIG. 2J). Other branched heteropolysaccharides such as arabinan, xylan, arabinoxylan, and xyloglucan were successfully dissociated and similarly generated several isomers per DP (FIGS. 2J-2M). The depolymerization of such diverse structures further demonstrates the method's reactivity towards pentose containing polysaccharides. Unexpectedly, amylopectin, another branched heteropolysaccharide containing an α(1→4) linked glucose backbone with α(1→4,6) bisecting glucose branches produced only one structure per DP, which may be due to the high oxidative lability that has been reported for (1→6) linkages (FIG. 2N).³³

Polysaccharides containing modified monosaccharide residues were also examined. Xylan was a β(1→4)-linked xylose polysaccharide with terminal 4-O-methyl glucuronic acid (GlcAOMe) residues. Under FITDOG conditions, several oligosaccharides were generated with unique compositions including 3Pent:1GlcAOMe isomers (13.06, 14.00, 14.27, and 16.47 min), 4Pent:1GlcAOMe isomers (17.68, 18.87, and 19.55 min), 5Pent:1GlcAOMe isomers (20.93, 22.82, 23.13, and 23.46 min), 6Pent:1GlcAOMe isomers (24.51, 26.88, and 27.34 min), and 7Pent:1GlcAOMe isomers (27.61 and 29.69 min). The presence of these oligosaccharides indicated that O-methylation on the terminal glucuronic acid was preserved after FITDOG treatment (FIG. 2J).

Example 3. Identification of Polysaccharides Through Oligosaccharide Fingerprinting

With the unique oligosaccharide compositions for each polysaccharide, we examined whether the oligosaccharides could be used as diagnostic markers for polysaccharide identification in complicated mixtures such as those found in common foods. The oligosaccharide products were used as unique identifiers for the respective parent polysaccharides. An oligosaccharide reference library was created from the polysaccharide standards with nearly 400 unique oligosaccharides. A pool containing all the oligosaccharides was run alongside unknown samples for retention time alignment (FIG. 3A).

Wheat and oat bran were selected to validate the oligosaccharide fingerprinting method as they were known to contain large amounts of non-starch polysaccharides including arabinoxylans³⁴, mixed linkage β-glucans³⁵, and cellulose³⁶. Oat and wheat bran each produced over 50 distinct oligosaccharides with a large fraction matching entries in the reference library (FIGS. 3B and 3C).

To determine the polysaccharide compositions of the two brans, we used an approach that was analogous to peptide fingerprinting for protein identification. The major caveat is that proteins are composed of a specified length of polypeptides, while polysaccharides are composed of a distribution of saccharide lengths. In this approach, we utilized the number of oligosaccharides produced in the individual standards to determine the fraction of the polysaccharide products that are observed in the mixture. The number of oligosaccharides produced varied across each standard polysaccharide. For cellulose, only four oligosaccharides were observed in our chromatographic window, while for amylopectin over 20 oligosaccharides were observed, and for arabinoxylan over 40 oligosaccharides were observed. We proposed that the number of oligosaccharide peaks found in the mixture can be represented as a fraction of those found in the pure standard and be used as a “percent coverage”. The coverage is expected to be higher if the oligosaccharide count in the sample is closer to the number of oligosaccharides produced in the standard.

Based on the analysis, wheat bran was found to contain amylose/amylopectin (20 peaks, 100% coverage), cellulose (3 peaks, 75%), β-glucan (11 peaks, 34%) and lichenan (9 peaks (24%). Oat bran was composed of amylose/amylopectin (20 peaks, 100% coverage), cellulose (3 peaks, 75%), β-glucan (12 peaks, 38%) and lichenan (9 peaks 24%). Oat bran also contained arabinoxylan (5 peaks, 20%) and xylan (7 peaks, 32%). Interestingly, oat bran contained unmatched pentose oligomers (3 peaks) and mixed hexose and pentose containing oligomers (7 peaks) suggesting a polysaccharide that had not yet been identified. Nonetheless, the results matched the general expected polysaccharide composition of the brans.³⁴⁻³⁶

Example 4. Production of Xyloglucan Resembling Oligosaccharides

Xyloglucan polysaccharide was extracted from tamarind and underwent the FITDOG process to produce oligosaccharides that resemble its structure.²⁷ Tamarind xyloglucan is known to comprise a β1,4 glucose backbone with frequent single unit branches of α1,6 xylose that can occasionally be further attached to a single β1,2 linked galactose endcap. Xyloglucan from other sources may contain a single fucose residue α1,2 linked to the galactose. In other sources of xyloglucan, arabinose can be α1,2 linked to the xylose residue. Thusly, xyloglucan resembling oligosaccharides are those that can be described by chains of 3-30 monosaccharides with a fundamental structure that contains a β1,4-linked glucose and between 1 and n branching xylose residues, where n is the number of glucoses in the oligosaccharide. The xylose can be linked to any glucose through an α1,6 linkage. The xylose may be, but is not required to be, further linked to a galactose residue primarily through a β1,2 linkage. Additionally, the xylose may be, but is not required to be, further linked to an arabinose residue primarily through an α1,2 linkage. The galactose may be, but is not required to be, further linked to a fucose residue primarily through an α1,2 linkage. An example of xyloglucan structure is shown in FIG. 4.

The FITDOG process created over 45 oligosaccharides that do not exist naturally. The oligosaccharides observed by HPLC/Q-TOF MS are summarized in Table 4. The oligosaccharides observed ranged from a degree of polymerization (DP) of 3-9 and contained combinations of hexoses, which can be derived from glucose or galactose, and pentoses, which are derived from xylose. In Table 4, each subunit labeled “Hex” is independently selected from unsubstituted β1-linked Glu, 6-substituted β1-linked Glu (e.g., (Xylα1-6)Glu), and unsubstituted β1-linked Gal. Each unsubstituted β1-linked Glu or 6-substituted β1-linked Glu is linked to the 4 position of a neighboring Glu or 6-substituted Glu, and each Gal is linked to the 2-position of a neighboring Xyl. In Table 4, each subunit labeled “Pent” is independently selected from unsubstituted α-linked Xyl and 2-substituted α-linked Xyl (e.g., (Galβ1-2)Xyl).

TABLE 4 Retention Mass Neutral Time # (m/z) Mass (min) Composition 1 477.18 474.16 6.88 2Hex:1Pent 2 477.18 474.16 7.68 2Hex:1Pent 3 477.18 474.16 10.94 2Hex:1Pent 4 579.21 576.19 12.47 3Pent1Hex 5 609.22 606.20 13.71 2Hex:2Pent 6 639.23 636.21 12.21 3Hex:1Pent 7 639.23 636.21 13.03 3Hex:1Pent 8 639.23 636.21 15.72 3Hex:1Pent 9 639.23 636.21 16.66 3Hex:1Pent 10 639.23 636.21 17.32 3Hex:1Pent 11 771.28 768.25 14.92 3Hex:2Pent 12 771.27 768.25 15.15 3Hex:2Pent 13 771.27 768.25 18.13 3Hex:2Pent 14 771.27 768.25 18.65 3Hex:2Pent 15 801.29 798.26 16.84 4Hex:1Pent 16 801.29 798.26 17.94 4Hex:1Pent 17 801.29 798.26 21.76 4Hex:1Pent 18 801.29 798.26 22.86 4Hex:1Pent 19 801.29 798.26 23.17 4Hex:1Pent 20 903.32 900.30 20.34 3Hex:3Pent 21 933.33 930.31 16.00 4Hex:2Pent 22 933.33 930.31 18.78 4Hex:2Pent 23 933.33 930.31 19.20 4Hex:2Pent 24 933.33 930.31 19.61 4Hex:2Pent 25 933.33 930.31 22.84 4Hex:2Pent 26 933.33 930.31 24.50 4Hex:2Pent 27 933.33 930.31 25.19 4Hex:2Pent 28 933.33 930.31 27.07 4Hex:2Pent 29 1065.36 1062.35 18.18 4Hex:3Pent 30 1065.36 1062.35 20.70 4Hex:3Pent 31 1065.36 1062.35 20.95 4Hex:3Pent 32 1065.36 1062.35 26.27 4Hex:3Pent 33 1065.36 1062.35 27.35 4Hex:3Pent 34 1095.38 1092.36 18.98 5Hex:2Pent 35 1095.38 1092.36 19.94 5Hex:2Pent 36 1095.38 1092.36 23.14 5Hex:2Pent 37 1095.38 1092.36 23.74 5Hex:2Pent 38 1095.38 1092.36 24.78 5Hex:2Pent 39 1095.38 1092.36 25.44 5Hex:2Pent 40 1227.43 1224.40 21.27 5Hex:3Pent 41 1227.43 1224.40 24.96 5Hex:3Pent 42 1227.43 1224.40 25.81 5Hex:3Pent 43 1257.44 1254.41 22.74 6Hex:2Pent 44 1389.47 1386.45 26.47 6Hex:3Pent 45 1389.47 1386.45 26.73 6Hex:3Pent 46 1389.47 1386.45 27.33 6Hex:3Pent

The oligosaccharide fragmentation was used in the elucidation of the oligosaccharide secondary structure. The sodium borohydrate reduction was used to reduce the aldehyde to an alditol, which can be observed as an increased m/z 2.014 in the fragmentation spectrum. This additional mass allowed the reducing end residue to be clearly identified and provided additional evidence for determining regiospecific monosaccharide arrangements.

An HPLC-MS chromatogram of the peaks described in Table 4 is shown in FIG. 5. The mixture of oligosaccharides were analyzed for broad attributes including monosaccharide and glycosidic linkage analysis. The monosaccharide analysis confirmed that the FITDOG process did not drastically change the monosaccharide composition of the polysaccharide during processing. The monosaccharide analysis showed that glucose, galactose, and xylose were the three major building blocks of the xyloglucan-resembling oligosaccharides. The glycosidic linkage analysis of the pool further confirmed that the FITDOG process did not greatly change alter the composition. As expected, terminal glucose, 4-linked glucose, 4,6-branched glucose, terminal xylose, 2-linked xylose, and terminal galactose were the major glycosidic linkages. The observed monosaccharide and glycosidic linkage analyses was used alongside the MS/MS annotations to deduce absolute oligosaccharide structure.

FIG. 6A shows the annotated mass spectrum of a smaller oligosaccharide with a DP of three. This oligosaccharide corresponded with oligosaccharide #3 from Table 4. The fragment m/z 345.14 confirmed that there were two hexoses in the backbone of the oligosaccharides. This was further characterized as a β1,4 glucose backbone because the 4-linked glucose was the only linked hexose to be found from the glycosidic linkage analysis. Furthermore, the fragment m/z 315.13 showed a pentose moiety was linked to the reducing end glucose. Xylose was the primary pentose in the monosaccharide analysis and is known to be linked directly to the glucose backbone through an β1,6 glycosidic bond in xyloglucan.

The annotated tandem mass spectrum shown in FIG. 6B corresponds to oligosaccharide #12 in Table 4, which has a DP of five. Again, the fragment m/z 345.14 showed a two-hexose backbone corresponding to two β1,4 linked glucose residues, where the m/z 315.13 corresponded to a xylose on the reducing end glucose. The fragment m/z 609.22 corresponded to xyloses attached to two glucose. A lack of a peak at m/z 265.08 indicates that the two pentoses are not linked together and must be situated with one pentose on each hexose. Furthermore, a peak with an m/z 507.17, which corresponds to three hexoses, was not observed. This shows that the three hexoses are not linked together, and one must be linked to one of the pentose residues. Lastly, the regio-location of this hexose was found to be located on the xylose residue that extends from the non-reducing hexose residue by the fragment m/z 457.15, which corresponds to one pentose, and two hexoses without a reducing end. The xylose capping hexose was determined to be a galactose based upon the terminal galactose observed in the glycosidic linkage analysis. Again, this oligosaccharide structure was further confirmed by comparison with the known structure of xyloglucan, where xylose can be α1,6 linked to terminal galactose residues.

The annotated fragmentation spectrum in FIG. 6C corresponds to oligosaccharide #20 in Table 4, which is a highly branched DP six oligosaccharide. Several key fragments aided in the elucidation of this oligosaccharide. For example, fragments with m/z 295.10 and m/z 315.13 correspond to a non-reducing end hexose linked to a pentose and a reducing end hexose linked to a pentose, respectively. The absence of peaks at m/z 265.08 and m/z 397.12, which correspond to two pentose and three pentoses, respectively, indicates that none of the pentoses are linked together and must all be exclusively linked to hexose residues. Furthermore, the peak m/z 589.20 corresponds to two non-reducing hexoses and two pentoses, while m/z 609.32 corresponds to two hexoses with a reducing end and two pentoses. These two peaks together suggest that there is a three-hexose backbone with each hexose containing a pentose branch. With knowledge of the monosaccharide composition and glycosidic linkages, it can be concluded that this peak corresponds to an oligosaccharide with three β1,4 linked glucose residues, each with their own β1,6 branched xylose residue.

The annotated fragmentation spectrum in FIG. 6D corresponds to oligosaccharide #42 from Table 4, which is highly branched and has an DP of eight. The backbone of this oligosaccharide can be deduced from the peak at m/z 831.29, which represents a backbone with five hexoses and a reducing end. This oligosaccharide lacks a peak at m/z 315.13, which corresponds to one reducing end hexoses with a pentose. This means that the oligosaccharide lacks a pentose branching from the reducing end hexose. The peak m/z 883.29, which represents three hexoses non-reducing end hexoses and three pentoses, and further corroborates the lack of a pentose on the reducing end. Furthermore, peak m/z 883.29 suggests that all three pentoses are linked to adjacent hexose residues. Lastly, the pentose regio-location can be confined to the 2^(nd), 3^(rd), and 4^(th) hexoses from the reducing end by the presence of the peak m/z 477.21, which corresponds to two hexoses with a reducing end and a pentose. The peak m/z 477.21 can be deduced as representing a two-hexose backbone with the pentose linked to the non-reducing hexose due to the above-explained evidence for pentoses not being linked to the reducing end. This oligosaccharide was therefore determined to contain five β1,4 linked glucoses, with the three middle glucoses containing β1,6 branched xylose residues.

The identities of compounds corresponding to peaks in Table 4 are shown below. Peaks are grouped together where an individual oligosaccharide could not be tied to a specific peak, but where groups of oligosaccharides having the same mass could be tied to groups of peaks.

 1 ((Galβ1-2)Xylα1-6)Glu  2 (Xylα1-6)Glcβ1-4Glu  3 Gluβ1-4(Xylα1-6)Glu  5 (Xylα1-6)Gluβ1-4(Xylα1-6)Glu  6 Gluβ1-4((Galβ1-2)Xylα1-6)Glu  7 ((Galβ1-2)Xylα1-6)Gluβ1-4Glu  8 Gluβ1-4Gluβ1-4(Xylα1-6)Glu  9 (Xylα1-6)Gluβ1-4Gluβ1-4Glu 10 Gluβ1-4(Xylα1-6)Gluβ1-4Glu 11 (Xylα1-6)Gluβ1-4((Galβ1-2)Xylα1-6)Glu 12 ((Galβ1-2)Xylα1-6)Gluβ1-4(Xylα1-6)Glu 13 (Xylα1-6)Gluβ1-4Gluβ1-4(Xylα1-6)Glu 14 Gluβ1-4(Xylα1-6)Gluβ1-4(Xylα1-6)Glu 15 (Xylα1-6)Gluβ1-4(Xylα1-6)Gluβ1-4Glu 16 Gluβ1-4Gluβ1-4((Galβ1-2)Xylα1-6)Glu 17 Gluβ1-4((Galβ1-2)Xylα1-6)Gluβ1-4Glu 18 ((Galβ1-2)Xylα1-6)Gluβ1-4Glc1-4Glu 19 Gluβ1-4Gluβ1-4Gluβ1-4(Xylα1-6)Glu 20 Gluβ1-4(Xylα1-6)Gluβ1-4Gluβ1-4Glu 21 Gluβ1-4Gluβ1-4(Xylα1-6)Gluβ1-4Glu 22 (Xylα1-6)Gluβ1-4Gluβ1-4Gluβ1-4Glu 23 (Xylα1-6)Gluβ1-4(Xylα1-6)Gluβ1-4(Xylα1-6)Glu 24 ((Galβ1-2)Xylα1-6)Gluβ1-4((Galβ1-2)Xylα1-6)Glu 25, 26, 27, 28, 29, Gluβ1-4(Xylα1-6)Gluβ1-4((Galβ1-2)Xylα1-6)Glu, and 30 Gluβ1-4((Galβ1-2)Xylα1-6)Gluβ1-4(Xylα1-6)Glu, (Xylα1-6)Gluβ1-4Gluβ1-4((Galβ1-2)Xylα1-6)Glu, ((Galβ1-2)Xylα1-6)Gluβ1-4Gluβ1-4(Xylα1-6)Glu, ((Galβ1-2)Xylα1-6)Gluβ1-4(Xylα1-6)Glcβ1-4Glu, or (Xylα1-6)Gluβ1-4((Galβ1-2)Xylα1-6)Gluβ1-4 Glu 31, 32, 33, 34, Gluβ1-4Gluβ1-4(Xylα1-6)Gluβ1-4(Xylα1-6)Glu, and 35 Gluβ1-4(Xylα1-6)Gluβ1-4(Xylα1-6)Gluβ1-4Glu, (Xylα1-6)Gluβ1-4Gluβ1-4(Xylα1-6)Gluβ1-4Glu, Gluβ1-4(Xylα1-6)Gluβ1-4Gluβ1-4(Xylα1-6)Glu, or (Xylα1-6)Gluβ1-4Gluβ1-4Gluβ1-4(Xylα1-6)Glu

Example 5. Production and Characterization of Oligosaccharides from Homopolymer and Heteropolymer Polysaccharides

The monosaccharide composition of polysaccharides prior to FITDOG depolymerization is shown below in Table 5-1. The notation “--” represents a monosaccharide that exists an amount less than 2% of the total polymer weight.

TABLE 5-1 Glucose Galactose Mannose Arabinose Xylose Rhamnose GalA Fructose Others Galactan — 88.2 — 2.95 — 2.20 2.83 — 3.80 Lichenan 80.16 10.58 5.53 — — — — — 3.72 β-Glucan 95.97 — — — — — — 2.80 1.24 Glucomannan 34.6 — 60.9 — — — — 2.37 2.11 Galactomannan 23.3 — 73.2 — — — — — 3.52 Arabinan 13.3 — — 81.6 — — — — 5.15 Xylan — — — — 94.2 — — — 5.77 Arabinoxylan — — — 36.3 59.7 — — — 3.97

The glycosidic linkage composition of polysaccharides prior to FITDOG depolymerization is shown below in Table 5-2. The notation “--” represents a monosaccharide that exists an amount less than 2% of the total polymer weight.

TABLE 5-2 4-Gal T-Gal 4-Man T-Man 3-Glu 4-Glu T-Glu 4,6-Man Galactan 58.84 30.53 — — — — — — Lichenan — 26.25 — — 24.51 26.61 2.46 — Beta-glucan — — 3.19 — 31.24 56.80 3.84 — Glucomannan — — 80.46 3.06 — 13.66 — — Galactomannan — 17.17 72.18 — — — — 4.53 Arabinan 7.79 12.28 — — — — — — Xylan — 2.43 — — — — — — Arabinoxylan — — — — — — — — 3,4-P- T-P- 2-F- 3-P- 4-P-Xyl Xyl Xyl Ara Ara T-F-Ara Others Galactan — — — — — — 10.63 Lichenan — — — — — 3.80 16.37 Beta-glucan — — — — — — 4.93 Glucomannan — — — — — — 2.82 Galactomannan — — — — — — 6.11 Arabinan 27.84 18.60 — — 2.70 25.18 5.61 Xylan 80.06 — 6.55 3.03 — — 7.92 Arabinoxylan 34.59 11.73 — 2.43 2.55 43.05 5.65

Curdlan. Due to the structure of curdlan being a homopolysaccharide, meaning both the linkage and monomers are consistent and non-varied, only one isomer for each degree of polymerization (DP) is expected. Indeed, at least four oligosaccharides were produced without any isomers as shown in Table 5-3. Likely, oligosaccharides with higher degrees of polymerization were produced; however, they were not within the chromatographic range.

TABLE 5-3 Retention Mass Neutral Time Compo- # (m/z) Mass (min) sition Compound 1 507.19 504.17 12.57 3Hex Glcβ1-3Glcβ1-3Glc 2 669.25 666.22 19.28 4Hex Glcβ1-3Glcβ1-3Glcβ1-3Glc 3 831.30 828.27 26.44 5Hex Glcβ1-3[Glcβ1-3]₃Glc 4 993.35 990.33 40.09 6Hex Glcβ1-3[Glcβ1-3]₄Glc

Galactan. Due to galactan being a homopolysaccharide, only one isomer for each degree of polymerization (DP) is expected. Indeed, at least seven oligosaccharides were produced without any isomers (Table 5-4). Likely, oligosaccharides with higher degrees of polymerization were produced; however, they were not within the chromatographic range.

TABLE 5-4 Retention Mass Neutral Time Compo- # (m/z) Mass (min) sition Compound 1  507.19  504.17  2.34 3Hex Galβ1-4Galβ1-4Gal 2  669.25  666.22  7.50 4Hex Galβ1-4Galβ1-4Galβ1-4Gal 3  831.30  828.27 11.19 5Hex Galβ1-4[Galβ1-4]₃Gal 4  993.35  990.33 12.40 6Hex Galβ1-4[Galβ1-4]₄Gal 5 1155.40 1152.38 13.10 7Hex Galβ1-4[Galβ1-4]₅Gal 6 1317.46 1314.43 13.68 8Hex Galβ1-4[Galβ1-4]₆Gal 7 1479.51 1476.49 14.62 9Hex Galβ1-4[Galβ1-4]₇Gal

Mannan. Due to the structure of mannan being a homopolysaccharide, meaning both the linkage and monomers are consistent and non-varied, only one isomer for each degree of polymerization (DP) is expected. Indeed, at least seven oligosaccharides were produced without any isomers (Table 5-5). Likely, oligosaccharides with higher degrees of polymerization were produced; however, they were not within the chromatographic range.

TABLE 5-5 Reten- tion Mass Neutral Time Compo- # (m/z) Mass (min) sition 1  507.19  504.17  1.51 3Hex Manβ1-4Manβ1-4Man 2  669.25  666.22  3.51 4Hex Manβ1-4Manβ1-4Manβ1-4Man 3  831.30  828.27  9.15 5Hex Manβ1-4[Manβ1-4]₃Man 4  993.35  990.33 11.93 6Hex Manβ1-4[Manβ1-4]₄Man 5 1155.40 1152.38 13.10 7Hex Manβ1-4[Manβ1-4]₅Man 6 1317.46 1314.43 14.06 8Hex Manβ1-4[Manβ1-4]₆Man 7 1479.51 1476.49 14.75 9Hex Manβ1-4[Manβ1-4]₇Man

Cereal Beta-Glucan. The FITDOG process produced oligosaccharides that are consistent with the polysaccharide structure of β-glucans as described above. For example, a trisaccharide can have three possible structural combinations: Glcβ1-4Glcβ1-4Glc, Glcβ1-4Glcβ1-3Glc, Glcβ1-3Glcβ1-4Glc. Indeed, three isomers corresponding to the mass of three-hexoses were observed. Furthermore, the isomer at retention time (RT) 13.73 minutes corresponded with the same RT as a structure also found in cellulose, which can be identified as Glcβ1-4Glcβ1-4Glc. The peaks at RT 11.38 and 15.17 minutes were therefore assigned to the structures that contain single β1-3 bonds between either the first and second glucoses or between the second and third glucoses. Further confidence in the assignments was gained due to the lack of a fourth trisaccharide isomer, which is not expected to be in β-glucan, and would correspond with Glcβ1-3Glcβ1-3Glc, which was seen at RT 12.57 minutes in curdlan. Similar logic was used to confirm the creation of at least 22 oligosaccharides observed from the FITDOG degradation of cereal beta-glucan (Table 5-6). Multiple compounds are listed for peaks which could be not be tied definitively to a single oligosaccharide, but rather to groups of oligosaccharides having the same mass.

In Table 5-6, each subunit labeled “Hex” is independently selected from unsubstituted, β1-linked Glu which is linked to the 3-position or the 4-position of a neighboring unsubstituted Glu.

TABLE 5-6 Retention Mass Neutral Time # (m/z) Mass (min) Composition Compound  1  507.19  504.17 11.38 3Hex Glcβ1-4Glcβ1-3Glc or Glcβ1-3Glcβ1-4Glc  2  507.19  504.17 13.73 3Hex  3  507.19  504.17 15.17 3Hex Glcβ1-4Glcβ1-3Glc or Glcβ1-3Glcβ1-4Glc  4  669.25  666.22 17.46 4Hex Glcβ1-3Glcβ1-4Glc1-4Glc, Glcβ1-4Glcβ1-3Glc1-4Glc, or Glcβ1-4Glcβ1-4Glc1-3Glc  5  669.25  666.22 19.46 4Hex  6  669.25  666.22 19.70 4Hex Glcβ1-3Glcβ1-4Glc1-4Glc, Glcβ1-4Glcβ1-3Glc1-4Glc, or Glcβ1-4Glcβ1-4Glc1-3Glc  7  669.25  666.22 20.41 4Hex Glcβ1-3Glcβ1-4Glc1-4Glc, Glcβ1-4Glcβ1-3Glc1-4Glc, or Glcβ1-4Glcβ1-4Glc1-3Glc  8  831.30  828.27 22.69 5Hex Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-3Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-3Glc1-4Glc, or Glcβ1-4Glcβ1-4Glc1-4Glc1-3Glc  9  831.30  828.27 23.52 5Hex Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-3Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-3Glc1-4Glc, or Glcβ1-4Glcβ1-4Glc1-4Glc1-3Glc 10  831.30  828.27 24.92 5Hex 11  831.30  828.27 25.79 5Hex Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-3Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-3Glc1-4Glc, or Glcβ1-4Glcβ1-4Glc1-4Glc1-3Glc 12  831.30  828.27 26.70 5Hex Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-3Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-3Glc1-4Glc, or Glcβ1-4Glcβ1-4Glc1-4Glc1-3Glc 13  993.35  990.33 13.35 6Hex Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-3Glc1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-3Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-4Glc1-3Glc1-4Glc, Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc1-3Glc, Glcβ1-4Glcβ1-4Glc1-4Glc1-4Glc1-3Glc 14  993.35  990.33 22.79 6Hex Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-3Glc1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-3Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-4Glc1-3Glc1-4Glc, Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc1-3Glc, Glcβ1-4Glcβ1-4Glc1-4Glc1-4Glc1-3Glc 15  993.35  990.33 25.10 6Hex Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-3Glc1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-3Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-4Glc1-3Glc1-4Glc, Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc1-3Glc, Glcβ1-4Glcβ1-4Glc1-4Glc1-4Glc1-3Glc 16  993.35  990.33 28.87 6Hex Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-3Glc1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-3Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-4Glc1-3Glc1-4Glc, Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc1-3Glc, Glcβ1-4Glcβ1-4Glc1-4Glc1-4Glc1-3Glc 17  993.35  990.33 30.90 6Hex Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-3Glc1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-3Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-4Glc1-3Glc1-4Glc, Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc1-3Glc, Glcβ1-4Glcβ1-4Glc1-4Glc1-4Glc1-3Glc 18  993.35  990.33 39.26 6Hex 19  993.35  990.33 41.49 6Hex Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-3Glc1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-3Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-4Glc1-3Glc1-4Glc, Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc1-3Glc, Glcβ1-4Glcβ1-4Glc1-4Glc1-4Glc1-3Glc 20 1155.40 1152.38 26.05 7Hex 21 1155.40 1152.38 27.23 7Hex 22 1155.40 1152.38 27.47 7Hex 23 1155.40 1152.38 28.61 7Hex

Lichenan. This structural differences between lichenan homopolymer and β-glucan homopolymer, as described above, were reflected in the number of isomers observed in oligosaccharide products obtained from lichenan. For example, depolymerization of β-glucan cannot produce the tetrasaccharide Glcβ1-3Glcβ1-4Glc1-3Glc because there must be at least two β1-4 glucose residues between each β1-3 glucose residue. However, lichenan was found to have all of the same isomers as β-glucan plus one additional isomer, which must therefore be associated with the Glcβ1-3Glcβ1-4Glc1-3Glc oligosaccharide. Similar logic was used to confirm the creation of at least 42 other oligosaccharides (Table 5-7).

In Table 5-7, each subunit labeled “Hex” is independently selected from unsubstituted, β1-linked Glu which is linked to the 3-position or the 4-position of a neighboring unsubstituted Glu.

TABLE 5-7 Retention Mass Neutral Time Com- # (m/z) Mass (min) position 1 507.19 504.17 5.98 3Hex 2 507.19 504.17 11.58 3Hex 3 507.19 504.17 13.73 3Hex 4 507.19 504.17 15.24 3Hex 5 669.25 666.22 14.13 4Hex 6 669.25 666.22 17.58 4Hex 7 669.25 666.22 19.46 4Hex 8 669.25 666.22 19.70 4Hex 9 669.25 666.22 20.41 4Hex 10 831.30 828.27 12.65 5Hex 11 831.30 828.27 13.47 5Hex 12 831.30 828.27 15.45 5Hex 13 831.30 828.27 23.64 5Hex 14 831.30 828.27 24.92 5Hex 15 831.30 828.27 25.79 5Hex 16 831.30 828.27 26.59 5Hex 17 993.35 990.33 9.72 6Hex 18 993.35 990.33 10.28 6Hex 19 993.35 990.33 11.86 6Hex 20 993.35 990.33 14.38 6Hex 21 993.35 990.33 16.23 6Hex 22 993.35 990.33 16.61 6Hex 23 993.35 990.33 29.00 6Hex 24 993.35 990.33 30.90 6Hex 25 993.35 990.33 39.26 6Hex 26 993.35 990.33 40.70 6Hex 27 1155.40 1152.38 9.59 7Hex 28 1155.40 1152.38 9.99 7Hex 29 1155.40 1152.38 10.50 7Hex 30 1155.40 1152.38 11.19 7Hex 31 1155.40 1152.38 11.97 7Hex 32 1155.40 1152.38 12.24 7Hex 33 1155.40 1152.38 15.65 7Hex 34 1155.40 1152.38 17.24 7Hex 35 1155.40 1152.38 17.58 7Hex 36 1155.40 1152.38 19.55 7Hex 37 1317.46 1314.43 11.16 8Hex 38 1317.46 1314.43 16.70 8Hex 39 1317.46 1314.43 18.17 8Hex 40 1317.46 1314.43 20.18 8Hex 41 1479.51 1476.49 20.81 9Hex 42 1641.56 1638.54 23.51 10Hex 43 1644.58 1641.56 23.57 10Hex 44 1317.46 1314.43 18.17 8Hex 45 1317.46 1314.43 20.18 8Hex 46 1479.51 1476.49 20.81 9Hex 47 1644.58 1641.56 23.57 10Hex

Compound 6 in Table 5-7 was identified as Glcβ1-3Glcβ1-4Glc1-3Glc. Compounds 8 and 9 in Table 5-7 were determined to be Glcβ1-3Glcβ1-4Glc1-4Glc, Glcβ1-4Glcβ1-3Glc1-4Glc or Glcβ1-4Glcβ1-4Glc1-3Glc, although the correlation of the specific peak to the specific oligosaccharide was not yet determined. Compound 15 in Table 5-7 was determined to be Glcβ1-3Glcβ1-4Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-3Glc1-4Glc1-4Glc, Glcβ1-4Glcβ1-4Glc1-3Glc1-4Glc, or Glcβ1-4Glcβ1-4Glc1-4Glc1-3Glc.

Glucomannan. The amount of oligosaccharide isomers in products obtained from glucomannan can be described as (#of isomers=n²) where “n” equals the number of monomers. For example, a trisaccharide would have eight isomers, and a tetrasaccharide would have 16 isomers, etc. This was confirmed in the number of isomers created for each DP. At DP's larger than 5, less than the expected number of isomers were observed, which could be explained by low probabilities of long chains of a single repeating monomer, low sensitivity, or co-elution. Data for the mixture of observed compounds is shown in Table 5-8.

In Table 5-8, each subunit labeled “Hex” is independently selected from unsubstituted, β1-linked Glu or unsubstituted, β1-linked Man, each of which is linked to the 4-position of a neighboring unsubstituted Glu or unsubstituted Man.

TABLE 5-8 Retention Mass Neutral Time # (m/z) Mass (min) Composition 1 507.19 504.17 1.51 3Hex 2 507.19 504.17 1.97 3Hex 3 507.19 504.17 6.87 3Hex 4 507.19 504.17 8.71 3Hex 5 507.19 504.17 10.05 3Hex 6 507.19 504.17 10.98 3Hex 7 507.19 504.17 12.88 3Hex 8 507.19 504.17 13.51 3Hex 9 669.25 666.22 3.51 4Hex 10 669.25 666.22 5.91 4Hex 11 669.25 666.22 10.76 4Hex 12 669.25 666.22 10.97 4Hex 13 669.25 666.22 11.42 4Hex 14 669.25 666.22 11.85 4Hex 15 669.25 666.22 14.04 4Hex 16 669.25 666.22 15.07 4Hex 17 669.25 666.22 15.45 4Hex 18 669.25 666.22 15.90 4Hex 19 669.25 666.22 16.28 4Hex 20 669.25 666.22 16.40 4Hex 21 669.25 666.22 16.70 4Hex 22 669.25 666.22 17.26 4Hex 23 669.25 666.22 18.84 4Hex 24 669.25 666.22 19.48 4Hex 25 831.30 828.27 9.15 5Hex 26 831.30 828.27 9.46 5Hex 27 831.30 828.27 10.68 5Hex 28 831.30 828.27 12.85 5Hex 29 831.30 828.27 13.73 5Hex 30 831.30 828.27 13.81 5Hex 31 831.30 828.27 14.03 5Hex 32 831.30 828.27 14.81 5Hex 33 831.30 828.27 15.34 5Hex 34 831.30 828.27 15.85 5Hex 35 831.30 828.27 15.99 5Hex 36 831.30 828.27 16.32 5Hex 37 831.30 828.27 16.46 5Hex 38 831.30 828.27 16.62 5Hex 39 831.30 828.27 16.91 5Hex 40 831.30 828.27 17.65 5Hex 41 831.30 828.27 20.30 5Hex 42 831.30 828.27 20.55 5Hex 43 831.30 828.27 21.05 5Hex 44 831.30 828.27 21.41 5Hex 45 831.30 828.27 22.68 5Hex 46 831.30 828.27 23.09 5Hex 47 993.35 990.33 11.93 6Hex 48 993.35 990.33 12.46 6Hex 49 993.35 990.33 13.85 6Hex 50 993.35 990.33 14.38 6Hex 51 993.35 990.33 14.49 6Hex 52 993.35 990.33 15.19 6Hex 53 993.35 990.33 16.00 6Hex 54 993.35 990.33 16.19 6Hex 55 993.35 990.33 16.23 6Hex 56 993.35 990.33 16.47 6Hex 57 993.35 990.33 17.17 6Hex 58 993.35 990.33 17.32 6Hex 59 993.35 990.33 17.74 6Hex 60 993.35 990.33 18.04 6Hex 61 993.35 990.33 18.32 6Hex 62 993.35 990.33 18.70 6Hex 63 993.35 990.33 18.95 6Hex 64 993.35 990.33 19.73 6Hex 65 993.35 990.33 19.93 6Hex 66 993.35 990.33 20.39 6Hex 67 993.35 990.33 20.46 6Hex 68 993.35 990.33 21.04 6Hex 69 993.35 990.33 21.22 6Hex 70 993.35 990.33 22.71 6Hex 71 993.35 990.33 23.39 6Hex 72 993.35 990.33 24.38 6Hex 73 993.35 990.33 24.47 6Hex 74 993.35 990.33 24.82 6Hex 75 993.35 990.33 25.27 6Hex 76 993.35 990.33 25.78 6Hex 77 993.35 990.33 26.39 6Hex 78 993.35 990.33 27.44 6Hex 79 1155.40 1152.38 13.11 7Hex 80 1155.40 1152.38 13.65 7Hex 81 1155.40 1152.38 14.53 7Hex 82 1155.40 1152.38 15.49 7Hex 83 1155.40 1152.38 15.72 7Hex 84 1155.40 1152.38 17.15 7Hex 85 1155.40 1152.38 17.58 7Hex 86 1155.40 1152.38 17.64 7Hex 87 1155.40 1152.38 17.84 7Hex 88 1155.40 1152.38 18.14 7Hex 89 1155.40 1152.38 18.38 7Hex 90 1155.40 1152.38 18.52 7Hex 91 1155.40 1152.38 18.70 7Hex 92 1155.40 1152.38 18.87 7Hex 93 1155.40 1152.38 19.10 7Hex 94 1155.40 1152.38 19.67 7Hex 95 1155.40 1152.38 19.71 7Hex 96 1155.40 1152.38 19.95 7Hex 97 1155.40 1152.38 20.08 7Hex 98 1155.40 1152.38 20.24 7Hex 99 1155.40 1152.38 20.42 7Hex 100 1155.40 1152.38 20.68 7Hex 101 1155.40 1152.38 20.96 7Hex 102 1155.40 1152.38 21.16 7Hex 103 1155.40 1152.38 21.48 7Hex 104 1155.40 1152.38 21.59 7Hex 105 1155.40 1152.38 22.01 7Hex 106 1155.40 1152.38 22.21 7Hex 107 1155.40 1152.38 23.24 7Hex 108 1155.40 1152.38 23.70 7Hex 109 1155.40 1152.38 24.27 7Hex 110 1155.40 1152.38 24.62 7Hex 111 1155.40 1152.38 25.02 7Hex 112 1155.40 1152.38 25.56 7Hex 113 1155.40 1152.38 26.62 7Hex 114 1155.40 1152.38 27.83 7Hex 115 1155.40 1152.38 28.38 7Hex 116 1317.46 1314.43 15.14 8Hex 117 1317.46 1314.43 16.72 8Hex 118 1317.46 1314.43 17.49 8Hex 119 1317.46 1314.43 17.86 8Hex 120 1317.46 1314.43 18.14 8Hex 121 1317.46 1314.43 18.30 8Hex 122 1317.46 1314.43 18.54 8Hex 123 1317.46 1314.43 18.79 8Hex 124 1317.46 1314.43 18.97 8Hex 125 1317.46 1314.43 19.20 8Hex 126 1317.46 1314.43 19.45 8Hex 127 1317.46 1314.43 19.59 8Hex 128 1317.46 1314.43 19.96 8Hex 129 1317.46 1314.43 20.13 8Hex 130 1317.46 1314.43 20.38 8Hex 131 1317.46 1314.43 20.64 8Hex 132 1317.46 1314.43 20.78 8Hex 133 1317.46 1314.43 20.96 8Hex 134 1317.46 1314.43 21.30 8Hex 135 1317.46 1314.43 21.54 8Hex 136 1317.46 1314.43 21.95 8Hex 137 1317.46 1314.43 22.55 8Hex 138 1317.46 1314.43 22.68 8Hex 139 1317.46 1314.43 23.13 8Hex 140 1317.46 1314.43 23.36 8Hex 141 1317.46 1314.43 23.71 8Hex 142 1317.46 1314.43 24.47 8Hex 143 1317.46 1314.43 25.20 8Hex 144 1317.46 1314.43 25.69 8Hex 145 1317.46 1314.43 26.01 8Hex 146 1317.46 1314.43 26.49 8Hex 147 1317.46 1314.43 26.97 8Hex 148 1317.46 1314.43 27.44 8Hex 149 1479.51 1476.49 15.97 9Hex 150 1479.51 1476.49 20.50 9Hex 151 1479.51 1476.49 20.97 9Hex 152 1479.51 1476.49 21.70 9Hex 153 1479.51 1476.49 22.85 9Hex 154 1479.51 1476.49 23.90 9Hex 155 1479.51 1476.49 25.65 9Hex 156 1479.51 1476.49 26.69 9Hex 157 1641.58 1638.56 15.38 10Hex 158 1803.61 1800.59 15.93 11Hex 159 1965.67 1962.64 16.36 12Hex 160 2127.72 2124.70 16.82 13Hex 161 2289.77 2286.75 17.12 14Hex 162 2451.83 2448.80 17.54 15Hex 163 2613.88 2610.86 17.87 16Hex 164 2775.93 2772.91 18.18 17Hex

The identities of compounds corresponding to peaks in Table 5-8 are shown below. Peaks are grouped together where an individual oligosaccharide could not be tied to a specific peak, but where groups of oligosaccharides having the same mass could be tied to groups of peaks.

1 Manβ1-4Manβ1-4Man 2, 3, 4, 5, 6, and 7 Glcβ1-4Manβ1-4Man, Manβ1-4Glcβ1-4Man, Manβ1-4Manβ1-4Glc, Glcβ1-4Glcβ1-4Man, or Glcβ1-4Manβ1-4Glc, or Manβ1-4Glcβ1-4Glc 9 Manβ1-4Manβ1-4Manβ1-4Man 10, 11, 12, 13, 14, Glcβ1-4Manβ1-4Manβ1-4Man, Glcβ1-4Glcβ1-4Manβ1-4Man, 15, 16, 17, 18, 19, Glcβ1-4Manβ1-4Glcβ1-4Man, Glcβ1-4Manβ1-4Manβ1-4Glc, 20, 21, 22, and 23 Glcβ1-4Glcβ1-4Glcβ1-4Man, Glcβ1-4Glcβ1-4Manβ1-4Glc, Glcβ1-4Manβ1-4Glcβ1-4Glc, Manβ1-4Glcβ1-4Manβ1-4Man, Manβ1-4Manβ1-4Glcβ1-4Man, Manβ1-4Manβ1-4Manβ1-4Glc, Manβ1-4Glcβ1-4Glcβ1-4Man, Manβ1-4Glcβ1-4Manβ1-4Glc, Manβ1-4Manβ1-4Glcβ1-4Glc, or Manβ1-4Glcβ1-4Glcβ1-4Glc 27 Manβ1-4[Manβ1-4]₃Man 47 Manβ1-4[Manβ1-4]₄Man 79 Manβ1-4[Manβ1-4]₅Man

Galactomannan. Because galactomannan and mannan have the same β1-4 mannose backbone, the peaks corresponding to the non-branched backbone was easily identified by comparing the retention times of the peaks that appeared in both chromatograms. It was inferred that peaks that were only found in galactomannan and not mannan, contained at least one branch. For example, the trisaccharide Manβ1-4Manβ1-4Man at RT 1.51 minutes was found in both mannan and galactomannan, which confirmed the creation of this trisaccharide. Furthermore, galactomannan was expected to contain two additional isomers, (Galα1-6)Manβ1-4Man and Manβ1-4(Galα1-6)Man. Indeed, two additional isomers at RT 1.78 and 2.56 minutes were observed and can be described as the two previously mentioned isomers. Similar logic was used to confirm the creation of 47 other oligosaccharides (Table 5-9).

In Table 5-9, each subunit labeled “Hex” is independently selected from unsubstituted, β1-linked Man or β1-linked (Galα1-6)Man, each of which is linked to the 4-position of a neighboring unsubstituted Man or (Galα1-6)Man.

TABLE 5-9 Retention Mass Neutral Time Com- # (m/z) Mass (min) position 1 507.19 504.17 1.51 3Hex 2 507.19 504.17 1.78 3Hex 3 507.19 504.17 2.56 3Hex 4 669.25 666.22 3.51 4Hex 5 669.25 666.22 4.01 4Hex 6 669.25 666.22 4.41 4Hex 7 669.25 666.22 4.94 4Hex 8 669.25 666.22 8.34 4Hex 9 831.30 828.27 9.20 5Hex 10 831.30 828.27 9.50 5Hex 11 831.30 828.27 10.13 5Hex 12 831.30 828.27 10.54 5Hex 13 831.30 828.27 11.06 5Hex 14 831.30 828.27 11.25 5Hex 15 831.30 828.27 12.06 5Hex 16 831.30 828.27 12.91 5Hex 17 993.35 990.33 11.93 6Hex 18 993.35 990.33 12.02 6Hex 19 993.35 990.33 12.23 6Hex 20 993.35 990.33 12.40 6Hex 21 993.35 990.33 12.60 6Hex 22 993.35 990.33 12.86 6Hex 23 993.35 990.33 13.41 6Hex 24 993.35 990.33 13.64 6Hex 25 993.35 990.33 14.13 6Hex 26 1155.40 1152.38 13.11 7Hex 27 1155.40 1152.38 13.51 7Hex 28 1155.40 1152.38 13.57 7Hex 29 1155.40 1152.38 13.72 7Hex 30 1155.40 1152.38 14.12 7Hex 31 1155.40 1152.38 14.22 7Hex 32 1155.40 1152.38 14.38 7Hex 33 1155.40 1152.38 14.65 7Hex 34 1155.40 1152.38 14.93 7Hex 35 1155.40 1152.38 15.33 7Hex 36 1317.46 1314.43 14.06 8Hex 37 1317.46 1314.43 14.34 8Hex 38 1317.46 1314.43 14.67 8Hex 39 1317.46 1314.43 14.90 8Hex 40 1317.46 1314.43 15.27 8Hex 41 1317.46 1314.43 15.58 8Hex 42 1317.46 1314.43 15.90 8Hex 43 1479.51 1476.49 14.80 9Hex 44 1479.51 1476.49 15.10 9Hex 45 1479.51 1476.49 15.97 9Hex 46 1479.51 1476.49 16.25 9Hex 47 1479.51 1476.49 16.55 9Hex

The identities of compounds corresponding to peaks in Table 5-9 are shown below. Peaks are grouped together where an individual oligosaccharide could not be tied to a specific peak, but where groups of oligosaccharides having the same mass could be tied to groups of peaks.

1 Manβ1-4Manβ1-4Man 2 and 3 (Galα1-6)Manβ1-4Man or Manβ1-4(Galα1-6)Man 4 Manβ1-4Manβ1-4Manβ1-4Man 5, 6, and 7 Manβ1-4(Galα1-6)Manβ1-4Man, (Galα1-6)Manβ1-4Manβ1-4Man, or Manβ1-4Manβ1-4(Galα1-6)Man 8 (Galα1-6)Manβ1-4(Galα1-6)Man 9 Manβ1-4[Manβ1-4]₃Man 10, 11, 12, and 13 (Galα1-6)Manβ1-4Manβ1-4Manβ1-4Man, Manβ1-4(Galα1-6)Manβ1-4Manβ1-4Man, Manβ1-4Manβ1-4(Galα1-6)Manβ1-4Man, or Manβ1-4Manβ1-4Manβ1-4(Galα1-6)Man 14, 15, and 16 (Galα1-6)Manβ1-4(Galα1-6)Manβ1-4Man, (Galα1-6)Manβ1-4Manβ1-4(Galα1-6)Man, or (Galα1-6)Manβ1-4(Galal-6)Manβ1-4Man 17 Manβ1-4[Manβ1-4]₄Man 18, 19, 20, 21, (Galα1-6)Manβ1-4Manβ1-4Manβ1-4Manβ1-4Man, and 22 Manβ1-4(Galα1-6)Manβ1-4Manβ1-4Manβ1-4Man, Manβ1-4Manβ1-4(Galα1-6)Manβ1-4Manβ1-4Man, Manβ1-4Manβ1-4Manβ1-4(Galα1-6)Manβ1-4Man, or Manβ1-4Manβ1-4Manβ1-4Manβ1-4(Galα1-6)Man 27, 28, 29, 30, 31, (Galα1-6)Manβ1-4Manβ1-4Manβ1-4Manβ1-4Manβ1-4Man, and 32 Manβ1-4(Galα1-6)Manβ1-4Manβ1-4Manβ1-4Manβ1-4Man, Manβ1-4Manβ1-4(Galα1-6)Manβ1-4Manβ1-4Manβ1-4Man, Manβ1-4Manβ1-4Manβ1-4(Galα1-6)Manβ1-4Manβ1-4Man, Manβ1-4Manβ1-4Manβ1-4Manβ1-4(Galα1-6)Manβ1-4Man, or Manβ1-4Manβ1-4Manβ1-4Manβ1-4Manβ1-4(Galα1-6)Man 26 Manβ1-4[Manβ1-4]₅Man 36 Manβ1-4[Manβ1-4]₆Man 43 Manβ1-4[Manβ1-4]₇Man

Xylan. The mass heterogeneity between xylose and glucuronic acid residues in xylan provided for convenient determination of linear and branched structures. For example, the tetrasaccharide Xylβ1-4Xylβ1-4Xylβ1-4Xyl was found to have a mass of 546.18 daltons, and the three possible branched trisaccharides had a mass of 604.19 daltons and were observed at RT 14.00, 15.33, and 16.47 minutes. Similar logic was used to confirm the creation of 27 other oligosaccharides (Table 5-10).

In Table 5-10, each subunit labeled “Pent” is independently selected from unsubstituted, β1-linked Xyl or β1-linked (GlcAOMeα1-2)Xyl, each of which is linked to the 4-position of a neighboring unsubstituted Xyl or (GlcAOMeα1-2)Xyl. Each “GlcAOMe” represents a glucuronic acid, which is methylated at the 4-O position.

TABLE 5-10 Retention Mass Neutral Time # (m/z) Mass (min) Composition 1 417.16 414.14 9.83 3Pent 2 549.20 546.18 15.33 4Pent 3 607.21 604.19 14.00 3Pent:1GlcAOMe 4 607.21 604.19 14.27 3Pent:1GlcAOMe 5 607.21 604.19 16.47 3Pent:1GlcAOMe 6 681.25 678.22 18.79 5Pent 7 739.26 736.23 17.68 4Pent: 1GlcAOMe 8 739.26 736.23 18.87 4Pent: 1GlcAOMe 9 739.26 736.23 19.55 4Pent: 1GlcAOMe 10 739.26 736.23 20.66 4Pent: 1GlcAOMe 11 813.29 810.26 21.92 6Pent 12 871.30 868.27 20.93 5Pent: 1GlcAOMe 13 871.30 868.27 22.82 5Pent: 1GlcAOMe 14 871.30 868.27 23.13 5Pent: 1GlcAOMe 15 871.30 868.27 23.46 5Pent: 1GlcAOMe 16 871.30 868.27 24.87 5Pent: 1GlcAOMe 17 945.33 942.31 25.73 7Pent 18 1003.34 1000.32 24.51 6Pent: 1GlcAOMe 19 1003.34 1000.32 26.88 6Pent: 1GlcAOMe 20 1003.34 1000.32 27.34 6Pent: 1GlcAOMe 21 1003.34 1000.32 27.52 6Pent: 1GlcAOMe 22 1003.34 1000.32 28.51 6Pent: 1GlcAOMe 23 1003.34 1000.32 29.39 6Pent: 1GlcAOMe 24 1077.37 1074.35 28.15 8Pent 25 1135.38 1132.36 27.61 7Pent: 1GlcAOMe 26 1135.38 1132.36 28.47 7Pent: 1GlcAOMe 27 1135.38 1132.36 29.69 7Pent: 1GlcAOMe

The identities of compounds corresponding to peaks in Table 5-10 are shown below. Peaks are grouped together where an individual oligosaccharide could not be tied to a specific peak, but where groups of oligosaccharides having the same mass could be tied to groups of peaks.

1 Xylβ1-4Xylβ1-4Xyl 2 Xylβ1-4Xylβ1-4Xylβ1-4Xyl 3, 4, (OMe-4-GlcAα1-2)Xylβ1-4Xylβ1-4Xyl, and 5 Xylβ1-4(OMe-4-GlcAα1-2)Xylβ1-4Xyl, Xylβ1-4Xylβ1-4(OMe-4-GlcAα1-2)Xyl 6 Xylβ1-4[Xylβ1-4]₃Xyl 7, 8, 9, (OMe-4-GlcAα1-2)Xylβ1-4Xylβ1-4Xylβ1-4Xyl, and 10 Xylβ1-4(OMe-4-GlcAα1-2)Xylβ1-4Xylβ1-4Xyl, Xylβ1-4Xylβ1-4(OMe-4-GlcAα1-2)Xylβ1-4Xyl, Xylβ1-4Xylβ1-4Xylβ1-4(OMe-4-GlcAα1-2)Xyl 11 Xylβ1-4[Xylβ1-4]₄Xyl 12, 13, (OMe-4-GlcAα1-2)Xylβ1-4[Xylβ1-4]₃Xyl, 14, 15, Xylβ1-4(OMe-4-GlcAα1-2)Xylβ1-4[Xylβ1-4]₂Xyl, and 16 Xylβ1-4Xylβ1-4(OMe-4-GlcAα1-2)Xylβ1-4Xylβ1-4Xyl, [Xylβ1-4]₃(OMe-4-GlcAα1-2)Xylβ1-4Xyl, [Xylβ1-4]₄(OMe-4-GlcAα1-2)Xyl 17 Xylβ1-4[Xylβ1-4]₅Xyl 18, 19, (OMe-4-GlcAα1-2)Xylβ1-4[Xylβ1-4]₄Xyl, 20, 21, Xylβ1-4(OMe-4-GlcAα1-2)Xylβ1-4[Xylβ1-4]₃Xyl, 22, Xylβ1-4Xylβ1-4(OMe-4-GlcAα1-2)Xylβ1-4[Xylβ1-4]₂Xyl, and 23 [Xylβ1-4]₃(OMe-4-GlcAα1-2)Xylβ1-4Xylβ1-4Xyl, [Xylβ1-4]₄(OMe-4-GlcAα1-2)Xylβ1-4Xyl, [Xylβ1-4]₅(OMe-4-GlcAα1-2)Xyl 24 Xylβ1-4]Xylβ1-4] ₆ Xyl

Arabinoxylan. Arabinoxylan was expected to have four trisaccharide isomers based on its polysaccharide structure. One isomer, RT 9.83 minutes, was also found in xylan, which confirms that the structure is comprised of only the backbone and no branching, Xylβ1-4Xylβ1-4Xyl. Furthermore, three more isomers at RT 2.00, 4.40, and 12.78 were observed and corresponded to the three expected structures (Araα1-3)Xylβ1-4Xyl, Xylβ1-4(Araα1-3)Xyl, ((Araα1-2)(Araα1-3))Xyl. Similar logic was used to confirm the production of 39 other oligosaccharides (Table 5-11).

In Table 5-11, each subunit labeled “Pent” is independently selected from unsubstituted, β1-linked Xyl, β1-linked (Araα1-3)Xyl, and β1-linked (Araα1-2)(Araα1-3)Xyl, each of which is linked to the 4-position of a neighboring Xyl, (Araα1-3)Xyl, or (Araα1-2)(Araα1-3)Xyl.

TABLE 5-11 Retention Mass Neutral Time Com- # (m/z) Mass (min) position 1 417.16 414.14 2.00 3Pent 2 417.16 414.14 4.40 3Pent 3 417.16 414.14 9.83 3Pent 4 417.16 414.14 12.78 3Pent 5 549.20 546.18 8.01 4Pent 6 549.20 546.18 10.19 4Pent 7 549.20 546.18 11.20 4Pent 8 549.20 546.18 11.58 4Pent 9 549.20 546.18 12.16 4Pent 10 549.20 546.18 12.38 4Pent 11 549.20 546.18 15.34 4Pent 12 681.25 678.22 12.39 5Pent 13 681.25 678.22 14.11 5Pent 14 681.25 678.22 14.36 5Pent 15 681.25 678.22 14.86 5Pent 16 681.25 678.22 15.82 5Pent 17 681.25 678.22 16.76 5Pent 18 681.25 678.22 17.15 5Pent 19 681.25 678.22 18.79 5Pent 20 813.29 810.26 14.03 6Pent 21 813.29 810.26 14.97 6Pent 22 813.29 810.26 15.36 6Pent 23 813.29 810.26 16.13 6Pent 24 813.29 810.26 17.21 6Pent 25 813.29 810.26 21.92 6Pent 26 945.33 942.31 16.61 7Pent 27 945.33 942.31 18.29 7Pent 28 945.33 942.31 22.54 7Pent 29 945.33 942.31 22.79 7Pent 30 945.33 942.31 25.73 7Pent 31 1077.37 1074.35 26.33 8Pent 32 1077.37 1074.35 26.83 8Pent 33 1077.37 1074.35 27.10 8Pent 34 1077.37 1074.35 27.49 8Pent 35 1077.37 1074.35 28.15 8Pent 36 1209.41 1206.39 26.37 9Pent 37 1209.41 1206.39 27.10 9Pent 38 1341.46 1338.43 27.10 10Pent 39 1473.50 1470.48 28.63 11Pent

The identities of compounds corresponding to peaks in Table 5-11 are shown below. Peaks are grouped together where an individual oligosaccharide could not be tied to a specific peak, but where groups of oligosaccharides having the same mass could be tied to groups of peaks.

1, 2, (Araα1-3)Xylβ1-4Xyl, Xylβ1-4(Araα1-3)Xyl, and 4 or ((Araα1-2)(Araα1-3))Xyl 3 Xylβ1-4Xylβ1-4Xyl 5, 6, 7, 8, (Araα1-3)Xylβ1-4Xylβ1-4Xyl, 9, and 10 Xylβ1-4(Araα1-3)Xylβ1-4Xyl, Xylβ1-4Xylβ1-4(Araα1-3)Xyl, (Araα1-3)Xylβ1-4(Araα1-3)Xyl, ((Araα1-2)(Araα1-3))Xylβ1-4Xyl, or Xylβ1-4((Araα1-2)(Araα1-3))Xyl 11 Xylβ1-4Xylβ1-4Xylβ1-4Xyl 19 Xylβ1-4[Xylβ1-4]₃Xyl 25 Xylβ1-4[Xylβ1-4]₄Xyl 30 Xylβ1-4[Xylβ1-4]₅Xyl 35 Xylβ1-4[Xylβ1-4]₆Xyl

Arabinan. The arabinan used for depolymerization was sourced from beet root. Arabinan was proposed to produce three trisaccharide isomers, Araα1-5Araα1-5Ara, (Araα1-3)Araα1-5Ara, and Araα1-5(Araα1-3)Ara. Indeed, three isomers were observed in the chromatogram at RT 2.97, 3.94, and 4.77 minutes. This trend continued for the four expected tetrasaccharide isomers, Araα1-5Araα1-5Araα1-5Ara, (Araα1-3)Araα1-5Ara1-5Ara, Araα1-5(Araα1-3)Ara1-5Ara, Araα1-5Ara1-5(Araα1-3)Ara. As expected, four isomers were found at RT 10.55, 11.03, 11.66, and 12.26 minutes. A total of 34 oligosaccharides were created from beetroot arabinan (Table 5-12).

In Table 5-12, each subunit labeled “Pent” is independently selected from unsubstituted, β1-linked Ara and β1-linked (Araα1-3)Ara, each of which is linked to the 5-position of a neighboring Ara or (Araα1-3)Ara.

TABLE 5-12 Retention Mass Neutral Time Com- # (m/z) Mass (min) position 1 417.16 414.14 2.96 3Pent 2 417.16 414.14 3.94 3Pent 3 417.16 414.14 4.77 3Pent 4 549.20 546.18 10.55 4Pent 5 549.20 546.18 11.03 4Pent 6 549.20 546.18 11.66 4Pent 7 549.20 546.18 12.26 4Pent 8 681.25 678.22 13.52 5Pent 9 681.25 678.22 14.03 5Pent 10 681.25 678.22 14.21 5Pent 11 681.25 678.22 14.50 5Pent 12 681.25 678.22 14.98 5Pent 13 813.29 810.26 15.11 6Pent 14 813.29 810.26 15.57 6Pent 15 813.29 810.26 16.13 6Pent 16 813.29 810.26 16.32 6Pent 17 813.29 810.26 16.52 6Pent 18 945.33 942.31 17.26 7Pent 19 945.33 942.31 17.51 7Pent 20 945.33 942.31 17.78 7Pent 21 945.33 942.31 18.00 7Pent 22 945.33 942.31 18.20 7Pent 23 1077.37 1074.35 18.42 8Pent 24 1077.37 1074.35 19.13 8Pent 25 1077.37 1074.35 19.53 8Pent 26 1209.41 1206.39 19.75 9Pent 27 1209.41 1206.39 20.20 9Pent 28 1209.41 1206.39 20.50 9Pent 29 1209.41 1206.39 20.75 9Pent 30 1341.46 1338.43 21.37 10Pent 31 1341.46 1338.43 21.62 10Pent 32 1473.50 1470.48 22.32 11Pent 33 1605.54 1602.52 23.54 12Pent 34 1737.58 1734.56 25.02 13Pent

Compounds 1-3 in Table 5-12 were determined to be Araα1-5Araα1-5Ara, (Araα1-3)Araα1-5Ara, and Araα1-5(Araα1-3)Ara, although the specific oligosaccharide corresponding to each peak was not yet definitively determined. Compounds 4-7 were determined to be Araα1-5Araα1-5Araα1-5Ara, (Araα1-3)Araα1-5Ara1-5Ara, Araα1-5(Araα1-3)Ara1-5Ara, and Araα1-5Ara1-5(Araα1-3)Ara, although the specific oligosaccharide corresponding to each peak was not yet definitively determined.

Example 6. Strategies for Structural Characterization of Oligosaccharides

The structural characterization of oligosaccharides is complicated and not stream-lined; thus, new strategies are often developed as new types and classes of oligosaccharides are discovered. Oligosaccharides prepared according to the present disclosure fall into different categories, e.g., 1) oligosaccharides derived from homopolymeric polysaccharides, containing a single type of monosaccharide and a single type of glycosidic linkage (e.g., amylose, galactan, mannan, curdlan, and cellulose); 2) oligosaccharides derived from heteropolymeric polymeric polysaccharides, containing non-isomeric monomers of various molecular weights and different glycosidic linkages (xylan, and xyloglucan); 3) oligosaccharides derived from heteropolymeric polysaccharides, containing different types of iosomeric monomers having the same molecular weight and/or different types of glycosidic linkages (glucomannan, galactomannan, and arabinoxylan); and 4) oligosaccharides derived from homopolymeric polysaccharides, containing a single monosaccharide unit and different types of glycosidic linkages (arabinan, lichenin, and beta-glucan). A flowchart (FIG. 7) was created to match oligosaccharide components with the respective logic that can be applied for their characterization. Examples are described below.

Strategy 1: Structural Characterization of Homopolymers

Homopolymers are the simplest oligosaccharides to characterize. Homopolymers were identified by the presence of a single monomer unit, when determined by a monosaccharide analysis, and only terminal and one type of linear linkage, when determined by a glycosidic linkage analysis. Once a homopolymer was determined, oligosaccharide analysis by HPLC-MS was performed to determine the degree of polymerization. For example, galactan contained 88% galactose (Table 5-1) and only two major glycosidic linkages, 58.84% 4-linked and 30.53% terminal (Table 5-2), which indicates it is a homopolymer. The HPLC-MS oligosaccharide analysis showed seven peaks corresponding to DPs of 3-9. Thus, the oligosaccharides were assigned to be polymers of Galβ1-4Gal (Table 5-4).

Strategy 2: Structural Characterization of Heteropolymers with Hetero-Mass Monomers

Heteropolymers with hetero-mass monomers can be the most structurally complicated oligosaccharides, however, the heterogeneity in mass results in oligosaccharides that can be differentiated by fragmentation in a mass spectrometer. Xyloglucan is an example of a heteropolymer with hetero-mass monomers because it contains glucose and galactose (180.16 Da) and xylose (150.13 Da). A full description of characterization with fragmentation-based logic flows is shown in Example 4 and FIG. 6.

Strategy 3: Structural Characterization of Heteropolymers with Homo-Mass Monomers

Heteropolymers with homo-mass monomers are the most complicated oligosaccharides to structurally elucidate because of the high number of possible structures but also the lack of information that can be obtained by MS fragmentation. One strategy that can be used to characterize the oligosaccharides is by a grouping strategy where groups of oligosaccharide isomers are assigned to groups of chromatographic peaks with the same mass. This strategy is particularly useful when the number of isomers per DP is consistent with the number of possible isomers dictated by the monomeric and glycosidic linkage profiles. For example, this strategy was used with beta-glucan, which contains mixtures of β1-3 and β1-4 glucose polymers (Example 5, Table 5-2, 5-3). When grouping oligosaccharides is not sufficient, de novo characterization methods can be used for structural characterization as described below in Example 6, Strategy 4.

Strategy 4: De Novo and Non-Biased Characterization of Polymers

The structures of oligosaccharide products produced herein that cannot be described by logic strategies 1-3, were and/or are characterized, according to the following de novo approach.

Fractionation of Oligosaccharides

Fractionation and detection of oligosaccharides was performed on an Agilent 1260 Infinity II series HPLC coupled to an Agilent 6530 Q-TOF mass spectrometer and a Teledyne Isco Foxy 200 fraction collector. Oligosaccharides were first separated on a 150 mm×4.6 mm Hypercarb column from Thermo Scientific with a 5 μm particle size. A binary gradient was employed and consisted of solvent A: (3% (v/v) acetonitrile/water+0.1% formic acid) and solvent B: (90% acetonitrile/water+0.1% formic acid). A 90-minute gradient with a flow rate of 1 ml/min was used for chromatographic separation: 5-12 B, 0-90 min; 12-99% B, 90-90.01 minutes; 99-99% B, 90.01-110 min; 99-5% B, 110-110.01 min; 5-5% B, 110.01-120 min. Post column, a 90:10 flow splitter partitioned the larger stream to the fraction collector and the smaller to the Q-TOF mass spectrometer. Data from the QTOF was collected in the positive mode and calibrated with internal calibrant ions ranging from m/z 118.086-2721.895. Drying gas was set to 150° C. and with a flow rate of 11 l/min. The fragment, skimmer, and Octupole 1 RF voltages were set to 75, 60, and 750 V, respectively. Fragmentation was performed at a rate of 1 spectra/second. The collision energy was based upon the compound mass and expressed by the linear function (Collision Energy=1.3*(m/z)-3.5). Fractions were collected on 96-well plates at a rate of 30 seconds per fraction. Collected fractions were dried to completion under vacuum centrifugation and reconstituted in 100 μl of nano-pure water. A 10 μl aliquot was transferred to a separate 96-well plate for monosaccharide composition analysis, while the remaining 90 μl underwent glycosidic linkage analysis.

Monosaccharide Composition Analysis of Fractionated Oligosaccharides

Briefly, fractionated oligosaccharides underwent acid hydrolysis with 4 M TFA for 2 hours at 100° C. The samples were dried to completion by vacuum centrifugation. Samples and monosaccharide standards (0.001-100 μg/ml) underwent derivatization with 0.2 M PMP in methanol and 28% NH₄OH at 70° C. for 30 minutes. Derivatized products were dried to completion under vacuum centrifugation and reconstituted in nano-pure water. Excess PMP was removed with chloroform extraction. The aqueous layer was analyzed by an Agilent 1290 infinity II UHPLC coupled to an Agilent 6495A QqQ MS employing dynamic multiple reaction monitoring (dMRM) mode. An external standard curve comprised of monosaccharide standards (fructose, mannose, allose, glucose, galactose, rhamnose, fucose, ribose, xylose, arabinose, glucuronic acid, galacturonic acid, N-acetylglucosamine, N-acetylgalactosamine) ranging from 0.001-100 μg/ml was used for absolute quantitation of each monosaccharide in the fractions. Detailed descriptions of the method can be found in Xu et al.²⁵ and Amicucci et al.²⁴

Linkage Analysis of Fractionated Oligosaccharides

Glycosidic linkage analysis was analyzed in the manner of Galermo et al.²⁶ Briefly, fractionated oligosaccharides and a pool of oligosaccharide standards (2-O-(α-D-Mannosepyranosyl)-D-mannopyranose, 1,4-D-xylobiose, 1,5-α-L-arabinotriose, 1,3-α-1,6-α-D-mannotriose, isomaltotriose, 4-O-(β-D-galactopyranosyl)-D-galactopyranose, lactose, 2′-fucosyllactose, nigerose, 3-O-(β-D-galactopyranosyl)-D-galactopyranose, 3-O-(α-D-mannopyranosyl)-D-mannopyranose, 1,4-β-D-mannotriose, maltohexaose, 1,6-α-D-mannotriose, and amylopectin obtained from carbosynth (Compton, U.K.), galactan (Lupin), 3′3-α-L-arabinofuranosylxylotetraose, sophorose, galactan, xyloglucan, mannan, galactomannan, beechwood xylan, arabinoxylan, arabinan, glucomannan, obtained from Megazyme (Chicago, Ill.); remaining partially permethylated monosaccharides were synthesized as described by Galermo et al⁵⁵) were reacted with saturated NaOH and iodomethane in DMSO. Residual NaOH and DMSO were removed by extraction with DCM and water. The DCM layer was dried to completion under vacuum centrifugation. Samples were hydrolyzed and derivatized in the same manner as the monosaccharide analysis. Samples did not undergo chloroform extraction and were reconstituted in 70% (v/v) methanol/water. Fractions were analyzed on an Agilent 1290 infinity II UHPLC coupled to an Agilent 6495A QqQ MS ran in multiple reaction monitoring (MRM) mode. The pool of oligosaccharide standards referenced above was used to assign the glycosidic linkages present based upon their m/z, fragmentation spectra, and retention time.

NMR Analysis of Fractionated Oligosaccharides

NMR spectra were recorded at 303 K on a Bruker AVANCE III 800 MHz spectrometer equipped with a 5-mm Bruker CPTCI cryoprobe. Samples were obtained by combining ten collections of fractionated oligosaccharides of the same components verified with HPLC-QTOF MS and MS/MS data. Based on the monosaccharide and linkage information, the most abundant oligosaccharides were selected for NMR analysis. Each of these selected pooled fractions were dried with vacuum centrifugation before being reconstituted in 0.4 mL of D₂O, and measured using 1D 1H (relaxation delay (D1) 2 s; number of scans (NS) 128), 13C NMR (D1 1.5 s; NS 6000-15000), and 2D 1H-1H COSY (D1 1.5 s; NS 8), 1H-13C HSQC (D1 2 s; NS 4), HMBC (D1 1.5 s; NS 16), and H2BC (D1 1.5 s; NS 16). The spectra were then processed with Bruker TopSpin 3.2 and analyzed with MestReNova. The experimental chemical shifts, along with the required monosaccharide and linkage data, were calculated using the CASPER program, where the oligosaccharide structures, including anomeric characters of linkages, were predicted with ranking scores.⁵⁶

Overview of Method

For the structural analysis of oligosaccharides that were not characterizable by the facile methods presented in Example 6, Strategies 1-3, we describe a method successfully used for their de novo elucidation herein. This strategy can also be employed to fully characterize the structure of any of the remaining uncharacterized oligosaccharides presented herein. In this strategy, the oligosaccharide products are chromatographically separated into pools of smaller numbers of compounds, many containing single unique structures. The LC eluant is split between the QTOF for MS and MS/MS and a 96 well collection plate. The QTOF MS and MS/MS mass spectra provides the structural information pertaining to monosaccharide compositions and the degree of oligosaccharide polymerization of the compounds collected at unique retention times. However, sole use of the QTOF MS and MS/MS mass spectra data may not identify monosaccharide constituents, linkages, or the anomeric character of the linkage (alpha versus beta). In such cases, the collected fractions (e.g., about 200) are further analyzed by rapid throughput monosaccharide and linkage analysis to provide nearly complete structures of each oligosaccharide component.

In one such example the galactomannan polysaccharide was used to illustrate the capabilities of these workflows. The LC-MS chromatogram yielded primarily hexose polymers with unknown monosaccharide compositions (combinations of galactose and mannose), linkage, or branching (FIG. 8A). Fractions from the LC-MS were collected in 30 second increments leading to a total of 192 fractions. Each fraction was analyzed for monosaccharide compositions as described above under “monosaccharide composition analysis of fractionated oligosaccharides”, and the results are shown in (FIG. 8B). The linkage information was similarly obtained using a separate workflow for comprehensive linkage analysis as described above under “linkage analysis of fractionated oligosaccharides”. When the three workflows (monosaccharide, glycosidic linkage, and oligosaccharide analysis) were integrated, they yielded structural information describing oligosaccharides with monosaccharide compositions and glycosidic linkage information.

To obtain the final structural feature, the anomeric character of the linkages, the isolated oligosaccharide fractions were selected for NMR analysis using 1H, 13C NMR and a combination of techniques including COSY, HSQC, HMBC, and H2BC described above under “NMR Analysis of Fractionated Oligosaccharides”, and the results are shown in FIG. 8C. The MS-obtained linkages and monosaccharide compositions greatly facilitated the NMR interpretation by limiting the resolution needed to determine the exact structures. This multi-workflow approach yielded absolute oligosaccharide structures, which were also used to recapitulate the parent polymeric structure (FIG. 8D).

Accordingly, the structures of any of the oligosaccharide peaks described in the tables (Tables 4, 5-3, 5-4, 5-5, 5-6, 5-7, 5-8, 5-9, 5-10, and 5-11) can be elucidated by the strategies presented in Strategies 1-4. Specifically, oligosaccharides 1-24 in Table 4 were characterized by Strategy 2, while oligosaccharides 25-35 in the same table were characterized by Strategy 3 and could be further characterized by Strategy 4. All oligosaccharides in Tables 5-3, 5-4, and 5-5 were characterized by Strategy 2. Oligosaccharides in Table 5-6 were characterized by Strategy 3 and can further be characterized by Strategy 4. The structures of oligosaccharides 2, 5, 10, and 18 were characterized as homologous compounds to compounds identified in curdlan because they had the same mass and retention time. The oligosaccharides in Table 5-7 were characterized by Strategy 3, and can be further characterized by Strategy 4.

The oligosaccharides 1, 9, 27, 47, and 79 in Table 5-8 were characterized by their homology to structures found in mannan; oligosaccharides 2-7, and 10-23 were characterized by Strategy 3 and can be further characterized by Strategy 4; oligosaccharides 8, 24-26, 28-46, 48-78, and 80-164 can be characterized by Strategy 4.

Oligosaccharides 1, 4, 17, 26, 36, and 43 in Table 5-9 were characterized by their homology to the same structures in mannan; oligosaccharides 2, 3, 5-7, 10-16, 18-22, and 27-32 were characterized by Strategy 3 and can be further characterized by Strategy 4; oligosaccharides 23-25, 27-35, 37-42, and 44-47 can be characterized by Strategy 4.

Oligosaccharides 1, 2, 6, 11, 17, and 24 in Table 5-10, were characterized by Strategy 2; oligosaccharides 3-5, 7-10, 12-16, and 18-23, were characterized by Strategy 3 and can be further characterized by Strategy 4; oligosaccharides 25-27 can be characterized by Strategy 4.

Oligosaccharides 3, 11, 19, 25, 30, and 35 in Table 5-11 were characterized by homology with structures found in xylan; oligosaccharides 1, 2, 4, and 5-10, were characterized by Strategy 3 and can be further characterized by Strategy 4; oligosaccharides 12-18, 20-24, 26-29, 31-34 can be characterized by Strategy 4.

Oligosaccharides 1-3, and 4-7, in Table 5-12 were characterized by Strategy 3 and can be further characterized by Strategy 4; oligosaccharides 8-34 can be characterized by Strategy 4.

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Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity and understanding, one of skill in the art will appreciate that certain changes and modifications may be practiced with the scope of the appended claims. In addition, each reference provided herein is incorporated by reference in its entirety to the same extent as if each reference was individually incorporated by reference. 

What is claimed is:
 1. A synthetic oligosaccharide comprising a backbone containing glucose monomers, wherein each glucose monomer is optionally bonded to a pendant xylose monomer, and wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to
 30. 2. The synthetic oligosaccharide of claim 1, wherein the glucose monomers in the backbone are β1-4 linked glucose monomers.
 3. The synthetic oligosaccharide of claim 1 or claim 2, wherein each pendant xylose monomer is bonded to a glucose monomer in the backbone by an α1-6 linkage.
 4. The synthetic oligosaccharide of any one of claims 1-3, further comprising one galactose monomer bonded to one or more pendant xylose monomers.
 5. The synthetic oligosaccharide of claim 4, wherein each galactose monomer is bonded to the pendant xylose monomer via a β1-2 linkage.
 6. The synthetic oligosaccharide of claim 4 or claim 5, further comprising one fucose monomer bonded to one or more galactose monomers.
 7. The synthetic oligosaccharide of claim 6, wherein each fucose monomer is bonded to the galactose monomer via an α1-2 linkage.
 8. The synthetic oligosaccharide of any one of claims 1-3, further comprising one arabinose monomer bonded to one or more pendant xylose monomers.
 9. The synthetic oligosaccharide of claim 8, wherein the arabinose monomer is bonded to the pendant xylose monomer via an α1-2 linkage.
 10. The synthetic oligosaccharide of any one of claims 1-9, comprising 2 to 4 glucose monomer in the backbone, 1 to 2 pendant xylose monomers bonded to different glucose monomers in the backbone, and 0 to 2 galactose monomers bonded to different xylose monomers.
 11. A synthetic oligosaccharide comprising a backbone containing mannose monomers, wherein each mannose monomer is optionally bonded to a pendant galactose monomer, and wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to
 30. 12. The synthetic oligosaccharide of claim 11, wherein the mannose monomers in the backbone are β1-4 linked mannose monomers.
 13. The synthetic oligosaccharide of claim 11 or claim 12, wherein each pendant galactose monomer is bonded to a mannose monomer in the backbone by an α1-6 linkage.
 14. The synthetic oligosaccharide of any one of claims 11-13, wherein the comprises 2-9 mannose backbone monomers, and 0-9 pendant galactose monomers.
 15. A synthetic oligosaccharide comprising mannose monomers, glucose monomers, or a combination thereof, wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to
 30. 16. The synthetic oligosaccharide of claim 15, wherein the synthetic oligosaccharide is linear, and wherein the synthetic oligosaccharide comprises at least one mannose monomer and at least one glucose monomer.
 17. The synthetic oligosaccharide of claim 15 or claim 16, wherein the glucose monomers are β1-4 linked to glucose or mannose monomers, and the mannose monomers are β1-4 linked to mannose or glucose monomers.
 18. A synthetic oligosaccharide comprising a backbone containing arabinose monomers, wherein each arabinose monomer is optionally bonded to a pendant arabinose monomer, and wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to
 30. 19. The synthetic oligosaccharide of claim 18, wherein the arabinose monomers in the backbone are α1-5 linked arabinose monomers.
 20. The synthetic oligosaccharide of claim 19, wherein each pendant arabinose monomer is bonded to an arabinose monomer in the backbone by an α1-3 linkage.
 21. The synthetic oligosaccharide of claim 20, wherein each pendant arabinose monomer is optionally bonded to another arabinose monomer by an α1-3 or α1-5 linkage.
 22. The synthetic oligosaccharide of claim 18, wherein the arabinose monomers in the backbone are α1-3 linked arabinose monomers.
 23. The synthetic oligosaccharide of claim 22, wherein each pendant arabinose monomer is bonded to an arabinose monomer in the backbone by an α1-5 linkage.
 24. The synthetic oligosaccharide of claim 23, wherein each pendant arabinose monomer is optionally bonded to another arabinose monomer by an α1-3 or α1-5 linkage.
 25. A synthetic oligosaccharide comprising β1-4 linked glucose monomers and β1-3 linked glucose monomers, wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to
 30. 26. The synthetic oligosaccharide of claim 25, wherein each β1-4 linked glucose monomer is optionally bonded to another β1-4 linked glucose monomer or to a β1-3 linked glucose monomer and each β1-3 glucose monomer is optionally bonded to another β1-3 linked glucose monomer or to a β1-4 linked glucose monomer.
 27. A synthetic oligosaccharide comprising a backbone containing xylose monomers, wherein each xylose monomer is optionally bonded to a pendant arabinose monomer or a pendant (4-O methylated) glucuronic acid monomer, and wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to
 30. 28. The synthetic oligosaccharide of claim 27, wherein the xylose monomers in the backbone are β1-4 linked xylose monomers.
 29. The synthetic oligosaccharide of claim 28, wherein each pendant arabinose monomer is bonded to a backbone xylose monomer with an α1-2 or α1-3 linkage.
 30. The synthetic oligosaccharide of claim 27 or claim 28, wherein two pendant arabinose monomers are bonded to the same backbone xylose with an α1-2 and an α1-3 linkage.
 31. The synthetic oligosaccharide of claim 27 or claim 28, wherein each pendant 4-O-methylglucuronic acid monomer is bonded to a xylose monomer with an α1-2 linkage.
 32. The synthetic oligosaccharide of any one of claims 1-31, which is set forth in any one of Table 4, Table 5-3, Table 5-4, Table 5-5, Table 5-6, Table 5-7, Table 5-8, Table 5-9, Table 5-10, Table 5-11, or Table 5-12.
 33. The synthetic oligosaccharide of any one of claims 1-32, which is obtained from a plant-derived polysaccharide.
 34. The synthetic oligosaccharide of claim 33, wherein the plant-derived polysaccharide is derived from tamarind, konjac root, a lichen, or a cereal grain.
 35. A composition comprising two more different synthetic oligosaccharides according to any one of claims 1-34.
 36. A composition comprising two more different synthetic oligosaccharides, wherein the synthetic oligosaccharides are obtained via depolymerization of one or more polysaccharides.
 37. The composition of claim 36, wherein the each polysaccharide is independently selected from the group consisting of xyloglucan, curdlan, galactan, mannan, lichenan, β-glucan, glucomannan, galactomannan, arabinan, xylan, and arabinoxylan.
 38. The composition of any one of claims 35-37, wherein the amount of at least one of synthetic oligosaccharides is at least 5 mol %, based on the total amount of oligosaccharide in the composition.
 39. Use of a synthetic oligosaccharide according to any one of claims 1-34 or a composition according to any one of claims 35-38 as a synbiotic, a prebiotic, an immune modulator, a digestion aid, a food additive, a supplement, a pharmaceutical excipient, or an analytical standard. 